Rv1518 Resolved · high auto-curated
H37Rv Rv1518 · MTBC0 mtbc0_001625 ·
319 aa · 1719453–1720412 (+) ·
RefSeq NP_216034.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase |
| Revised (this work) | Glycosyltransferase. Pfam: Glyco_tranf_2_3 (PF13641.13), Glycos_transf_2 (PF00535.33). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLV9
SwissProt · reviewed
· Predicted
|
|---|---|
| UniProt name | Uncharacterized protein Rv1518 |
UniProt still lists this protein as Uncharacterized protein Rv1518; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| eggNOG description | Glycosyl transferase family 2 |
| Orthologous group | COG1215 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.222 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 11 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_tranf_2_3 | PF13641.13 | 1.3e-09 | 18–228 | Glycosyltransferase like family 2 |
Glycos_transf_2 | PF00535.33 | 6.4e-25 | 20–159 | Glycosyl transferase family 2 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1520 (sugar transferase), high confidence from genomic context alone (score 854 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1520 |
sugar transferase | 853 | 854 ctx | neighborhood:449 cooccurence:744 |
Rv1516c |
sugar transferase | 832 | 832 ctx | cooccurence:745 |
Rv1517 hyp |
hypothetical protein | 894 | 779 ctx | neighborhood:778 textmining:542 |
Rv1519 hyp |
hypothetical protein | 604 | 589 ctx | neighborhood:510 |
Rv1514c |
glycosyltransferase | 533 | 533 | |
Rv0112 gca |
GDP-mannose 4,6-dehydratase | 437 | 420 | |
Rv1515c hyp |
hypothetical protein | 404 | 401 ctx | neighborhood:401 |
Rv1525 wbbL2 |
rhamnosyl transferase WbbL | 750 | 268 | textmining:673 |
Rv1527c pks5 |
polyketide synthase | 641 | 249 | textmining:542 |
Rv1503c |
Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi | 622 | 232 | textmining:528 |
Rv1505c hyp |
hypothetical protein | 832 | 147 | textmining:812 |
Rv1522c mmpL12 |
transmembrane transport protein MmpL12 | 650 | 108 | textmining:624 |
Rv1528c papA4 |
polyketide synthase associated protein PapA | 644 | 78 | textmining:630 |
Rv1506c hyp |
hypothetical protein | 525 | 66 | textmining:513 |
Rv3673c |
membrane-anchored thioredoxin-like protein | 656 | 53 | textmining:652 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: glycosyltransferase
- Pfam (hmmscan --cut_ga): Glyco_tranf_2_3 PF13641.13 (E=1e-09), Glycos_transf_2 PF00535.33 (E=6e-25)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216034.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_tranf_2_3 (PF13641.13), Glycos_transf_2 (PF00535.33)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1215 - Curated reference: UniProt P9WLV9 (SwissProt, reviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
18 functional partner(s); context anchor
Rv1520 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001625|Rv1518| MVPGDASSVVSVNPAKPLISVCIPMYNNGATIERCLRSILEQEGVEFEIVVVDDDSSDDCAAIAATMLRPGDRLLRNEPRLGLNRNHNKCLEVARGGLIQFVHGDDRLLPGALQTLSRRFEDPSVGMAFAPRRVESDDIKWQQRYGRVHTRFRKLRDRNHGPSLVLQMVLHGAKENWIGEPTAVMFRRQLALDAGGFRTDIYQLVDVDFWLRLMLRSAVCFVPHELSVRRHTAATETTRVMATRRNVLDRQRILTWLIVDPLSPNSVRSAAALWWIPAWLAMIVEVAVLGPQRRTHLKALAPAPFREFAHARRQLPMAD