Rv1526c Resolved · high auto-curated
H37Rv Rv1526c · MTBC0 mtbc0_001633 ·
426 aa · 1730589–1731869 (-) ·
RefSeq NP_216042.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | glycosyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase |
| Revised (this work) | Glycosyltransferase. Pfam: Glyco_transf_28 (PF03033.27), EryCIII-like_C (PF06722.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLV1
SwissProt · reviewed
· Predicted
|
|---|---|
| UniProt name | Uncharacterized protein Rv1526c |
UniProt still lists this protein as Uncharacterized protein Rv1526c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversionG Carbohydrate transport and metabolism
|
|---|---|
| eggNOG description | COG1819 Glycosyl transferases, related to UDP-glucuronosyltransferase |
| Orthologous group | COG1819 |
| EC number |
EC 2.4.1.173
|
| KEGG orthology |
K05841
|
| CAZy family |
GT1
|
| Gene Ontology (14) |
GO:0003674, GO:0003824, GO:0005575, GO:0005622, GO:0005623, GO:0008194, GO:0016740, GO:0016757, GO:0043226, GO:0043227, GO:0043229, GO:0043231 +2 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 3.932 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 10 missense, 1 nonsense, 2 frameshift |
| Disruption | 3 distinct premature-stop/frameshift site(s); most common in 6.57% of strains (9540) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_transf_28 | PF03033.27 | 2.3e-08 | 3–50 | Glycosyltransferase family 28 N-terminal domain |
EryCIII-like_C | PF06722.19 | 1.4e-13 | 292–394 | Erythromycin biosynthesis protein CIII-like, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pks5 (polyketide synthase), high confidence from genomic context alone (score 872 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1527c pks5 |
polyketide synthase | 880 | 872 ctx | neighborhood:743 coexpression:448 |
Rv3820c papA2 |
trehalose-2-sulfate acyltransferase | 593 | 576 ctx | cooccurence:530 |
Rv3824c papA1 |
acyltransferase | 570 | 552 ctx | cooccurence:537 |
Rv1182 papA3 |
acyltransferase papA3 | 569 | 551 ctx | cooccurence:525 |
Rv0764c cyp51 |
lanosterol 14-alpha demethylase | 486 | 474 | |
Rv1851 ureF |
urease accessory protein UreF | 406 | 407 ctx | cooccurence:401 |
Rv0295c stf0 hyp |
hypothetical protein | 403 | 404 ctx | cooccurence:402 |
Rv2946c pks1 |
polyketide synthase | 425 | 394 | |
Rv2383c mbtB |
phenyloxazoline synthase | 504 | 337 | |
Rv1875 hyp |
hypothetical protein | 827 | 158 | textmining:803 |
Rv1937 |
oxygenase | 820 | 77 | textmining:814 |
Rv1728c hyp |
hypothetical protein | 804 | 50 | textmining:803 |
Rv0544c |
transmembrane protein | 552 | 47 | textmining:550 |
Rv3150 nuoF |
NADH-quinone oxidoreductase subunit F | 651 | 46 | textmining:650 |
Rv3003c ilvB1 |
acetolactate synthase large subunit IlvB | 626 | 42 | textmining:626 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: glycosyltransferase
- MTBC0 PGAP product: glycosyltransferase
- Pfam (hmmscan --cut_ga): Glyco_transf_28 PF03033.27 (E=2e-08), EryCIII-like_C PF06722.19 (E=1e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216042.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_transf_28 (PF03033.27), EryCIII-like_C (PF06722.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1819 - Curated reference: UniProt P9WLV1 (SwissProt, reviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
18 functional partner(s); context anchor
pks5 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001633|Rv1526c| MKFVLAVHGTRGDVEPCAAVGVELRRRGHAVHMAVPPNLIEFVESAGLTGVAYGPDSDEQINTVAAFVRNLTRAQNPLNLARAVKELFVEGWAEMGTTLTTLADGADLVMTGQTYHGVAANVAEYYDIPAAALHHFPMQVNGQIAIPSIPTPATLVRATMKVSWRLYAYVSKDADRAQRRELGLPPAPAPAVRRLAERGAPEIQAYDPVFFPGLAAEWSDRRPFVGPLTMELHSEPNEELESWIAAGTPPIYFGFGSTPVQTPVQTLAMISDVCAQLGERALIYSPAANSTRIRHADHVKRVGLVNYSTILPKCRAVVHHGGAGTTAAGLRAGMPTLILWDVADQPIWAGAVQRLKVGSAKRFTNITRGSLLKELRSILAPECAARAREISTRMTRPTAAVTAAADLLEATARQTPGSTPSSSPGR