wbbL2 Family assigned · low auto-curated
H37Rv Rv1525 · MTBC0 mtbc0_001632 ·
261 aa · 1729826–1730611 (+) ·
RefSeq NP_216041.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | rhamnosyl transferase WbbL |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase family 2 protein |
| Revised (this work) | Glycosyltransferase family 2 protein. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLV3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv1525 |
UniProt still lists this protein as Uncharacterized protein Rv1525; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | wbbL2 |
| eggNOG description | possibly involved in cell wall arabinogalactan linker formation uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall |
| Orthologous group | COG1216 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 5 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 1.40% of strains (2027) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: PPE8 (PPE family protein PPE8), high confidence from genomic context alone (score 751 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0355c PPE8 |
PPE family protein PPE8 | 751 | 751 ctx | cooccurence:749 |
Rv3347c PPE55 |
PPE family protein PPE55 | 749 | 750 ctx | cooccurence:748 |
Rv3350c PPE56 |
PPE family protein PPE56 | 749 | 749 ctx | cooccurence:747 |
Rv0304c PPE5 |
PPE family protein PPE5 | 748 | 749 ctx | cooccurence:746 |
Rv1917c PPE34 |
PPE family protein PPE34 | 742 | 743 ctx | cooccurence:740 |
Rv1524 |
glycosyltransferase | 751 | 742 ctx | neighborhood:670 |
Rv2490c PE_PGRS43 |
PE-PGRS family protein PE_PGRS43 | 739 | 740 ctx | cooccurence:739 |
Rv3343c PPE54 |
PPE family protein PPE54 | 736 | 737 ctx | cooccurence:733 |
Rv0341 iniB |
isoniazid inducible protein IniB | 810 | 735 ctx | cooccurence:714 |
Rv2209 |
integral membrane protein | 730 | 731 ctx | cooccurence:730 |
Rv1452c PE_PGRS28 |
PE-PGRS family protein PE_PGRS28 | 728 | 729 ctx | cooccurence:728 |
Rv1004c |
membrane protein | 726 | 727 ctx | cooccurence:726 |
Rv0872c PE_PGRS15 |
PE-PGRS family protein PE_PGRS15 | 720 | 721 ctx | cooccurence:720 |
Rv3879c espK |
ESX-1 secretion-associated protein EspK | 718 | 719 ctx | cooccurence:718 |
Rv0305c PPE6 |
PPE family protein PPE6 | 717 | 718 ctx | cooccurence:716 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: rhamnosyl transferase WbbL
- MTBC0 PGAP product: glycosyltransferase family 2 protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216041.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1216 - Curated reference: UniProt P9WLV3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
96 functional partner(s); context anchor
PPE8 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001632|Rv1525|wbbL2 MYAPLVSLMITVPVFGQHEYTHALVADLEREGADYLIVDNRGDYPRIGTERVSTPGENLGWAGGSELGFRLAFAEGYSHAMTLNNDTRVSKGFVAALLDSRLPADAGMVGPMFDVGFPFAVADEKPDAESYVPRARYRKVPAVEGTALVMSRDCWDAVGGMDLSTFGRYGWGLDLDLALRARKSGYGLYTTEMAYINHFGRKTANTHFGGHRYHWGASAAMIRGLRRTHGWPAAMGILREMGMAHHRKWHKSFPLTCPASC