Rv1524 Resolved · high auto-curated
H37Rv Rv1524 · MTBC0 mtbc0_001631 ·
414 aa · 1728535–1729779 (+) ·
RefSeq NP_216040.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | glycosyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase |
| Revised (this work) | Glycosyltransferase. Pfam: Glyco_transf_28 (PF03033.27), EryCIII-like_C (PF06722.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WN07
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized glycosyltransferase Rv1524 |
| EC (curated) |
EC 2.4.-.-
|
UniProt still lists this protein as Uncharacterized glycosyltransferase Rv1524; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversionG Carbohydrate transport and metabolism
|
|---|---|
| eggNOG description | COG1819 Glycosyl transferases, related to UDP-glucuronosyltransferase |
| Orthologous group | COG1819 |
| EC number |
EC 2.4.1.173
|
| KEGG orthology |
K05841
|
| CAZy family |
GT1
|
| Gene Ontology (14) |
GO:0003674, GO:0003824, GO:0005575, GO:0005622, GO:0005623, GO:0008194, GO:0016740, GO:0016757, GO:0043226, GO:0043227, GO:0043229, GO:0043231 +2 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.16 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 11 synonymous, 5 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.24% of strains (350) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_transf_28 | PF03033.27 | 1.9e-08 | 3–84 | Glycosyltransferase family 28 N-terminal domain |
EryCIII-like_C | PF06722.19 | 2.3e-13 | 295–396 | Erythromycin biosynthesis protein CIII-like, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1523 (methyltransferase), high confidence from genomic context alone (score 898 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1523 |
methyltransferase | 898 | 898 ctx | neighborhood:721 coexpression:651 |
Rv1525 wbbL2 |
rhamnosyl transferase WbbL | 751 | 742 ctx | neighborhood:670 |
Rv1182 papA3 |
acyltransferase papA3 | 636 | 621 ctx | cooccurence:608 |
Rv3824c papA1 |
acyltransferase | 627 | 612 ctx | cooccurence:600 |
Rv3820c papA2 |
trehalose-2-sulfate acyltransferase | 619 | 603 ctx | cooccurence:589 |
Rv1522c mmpL12 |
transmembrane transport protein MmpL12 | 526 | 509 ctx | neighborhood:497 |
Rv0295c stf0 hyp |
hypothetical protein | 498 | 499 ctx | cooccurence:493 |
Rv0764c cyp51 |
lanosterol 14-alpha demethylase | 489 | 477 | |
Rv1851 ureF |
urease accessory protein UreF | 421 | 422 ctx | cooccurence:413 |
Rv0063 |
oxidoreductase | 421 | 422 | |
Rv1181 pks4 |
polyketide beta-ketoacyl synthase | 430 | 402 | |
Rv2226 hyp |
hypothetical protein | 400 | 401 | |
Rv2946c pks1 |
polyketide synthase | 406 | 372 | |
Rv0519c |
membrane protein | 403 | 372 | |
Rv3351c hyp |
hypothetical protein | 902 | 277 | textmining:870 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: glycosyltransferase
- MTBC0 PGAP product: glycosyltransferase
- Pfam (hmmscan --cut_ga): Glyco_transf_28 PF03033.27 (E=2e-08), EryCIII-like_C PF06722.19 (E=2e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216040.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_transf_28 (PF03033.27), EryCIII-like_C (PF06722.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1819 - Curated reference: UniProt P9WN07 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
24 functional partner(s); context anchor
Rv1523 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001631|Rv1524| MKFVVASYGTRGDIEPCAAVGLELQRRGHDVCLAVPPNLIGFVETAGLSAVAYGSRDSQEQLDEQFLHNAWKLQNPIKLLREAMAPVTEGWAELSAMLTPVAAGADLLLTGQIYQEVVANVAEHHGIPLAALHFYPVRANGEIAFPARLPAPLVRSTITAIDWLYWRMTKGVEDAQRRELGLPKASTPAPRRMAVRGSLEIQAYDALCFPGLAAEWGGRRPFVGALTMESATDADDEVASWIAADTPPIYFGFGSMPIGSLADRVAMISAACAELGERALICSGPSDATGIPQFDHVKVVRVVSHAAVFPTCRAVVHHGGAGTTAAGLRAGIPTLILWVTSDQPIWAAQIKQLKVGRGRRFSSATKESLIADLRTILAPDYVTRAREIASRMTKPAASVTATADLLEDAARRAR