pknH Resolved · high auto-curated
H37Rv Rv1266c · MTBC0 mtbc0_001355 ·
550 aa · 1422404–1424056 (-) ·
RefSeq NP_215782.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | serine/threonine-protein kinase PknH |
|---|---|
| MTBC0 PGAP re-annotation | serine/threonine protein kinase PknH |
| Revised (this work) | Serine/threonine protein kinase PknH. Pfam: Pkinase (PF00069.32), PK_Tyr_Ser-Thr (PF07714.24), PknH_C (PF14032.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WI71
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Serine/threonine-protein kinase PknH |
| EC (curated) |
EC 2.7.11.1
|
| Curated function | May regulate bacterial growth in response to external signals to facilitate adaptation to the host environment. In vitro, phosphorylates several substrates such as EmbR, DevR (DosR), DacB1 and Rv0681. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K TranscriptionL Replication, recombination and repairT Signal transduction mechanisms
|
|---|---|
| Preferred name | pknH |
| eggNOG description | serine threonine protein kinase |
| Orthologous group | COG0515 |
| EC number |
EC 2.7.11.1
|
| KEGG orthology |
K08884, K12132
|
| Gene Ontology (105) |
GO:0003674, GO:0003824, GO:0004672, GO:0004674, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0005887, GO:0006355, GO:0006464, GO:0006468 +93 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.612 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 7 synonymous, 13 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.21% of strains (312) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Pkinase | PF00069.32 | 1.5e-31 | 10–154 | Protein kinase domain |
PK_Tyr_Ser-Thr | PF07714.24 | 6.2e-20 | 12–185 | Protein tyrosine and serine/threonine kinase |
PknH_C | PF14032.13 | 1.0e-62 | 361–546 | PknH-like extracellular domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pstP (phosphoserine/threonine phosphatase PstP), high confidence from genomic context alone (score 842 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0018c pstP |
phosphoserine/threonine phosphatase PstP | 888 | 842 ctx | cooccurence:761 |
Rv1267c embR |
transcriptional regulator EmbR | 956 | 762 | experimental:500 textmining:825 |
Rv1827 garA |
glycogen accumulation regulator GarA | 792 | 622 ctx | cooccurence:462 textmining:474 |
Rv0019c fhaB |
FHA domain-containing protein FhaB | 668 | 540 | |
Rv2031c hspX |
alpha-crystallin | 508 | 508 | experimental:500 |
Rv0020c fhaA |
FHA domain-containing protein FhaA | 712 | 479 | textmining:471 |
Rv0014c pknB |
serine/threonine-protein kinase PknB | 613 | 415 | |
Rv1747 |
ABC transporter ATP-binding protein/permease | 676 | 385 | textmining:496 |
Rv1364c |
sigma factor regulatory protein | 522 | 376 | |
Rv3080c pknK |
serine/threonine-protein kinase PknK | 512 | 347 | |
Rv3360 hyp |
hypothetical protein | 475 | 322 | |
Rv2914c pknI |
serine/threonine-protein kinase PknI | 510 | 317 | |
Rv2088 pknJ |
transmembrane serine/threonine-protein kinase PknJ | 485 | 284 | |
Rv1746 pknF |
serine/threonine-protein kinase PknF | 491 | 273 | |
Rv2145c wag31 |
cell wall synthesis protein Wag31 | 453 | 268 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: serine/threonine-protein kinase PknH
- MTBC0 PGAP product: serine/threonine protein kinase PknH
- Pfam (hmmscan --cut_ga): Pkinase PF00069.32 (E=1e-31), PK_Tyr_Ser-Thr PF07714.24 (E=6e-20), PknH_C PF14032.13 (E=1e-62)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215782.1)
- Domains: Pfam-A via hmmscan --cut_ga — Pkinase (PF00069.32), PK_Tyr_Ser-Thr (PF07714.24), PknH_C (PF14032.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0515 - Curated reference: UniProt P9WI71 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
26 functional partner(s); context anchor
pstP - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001355|Rv1266c|pknH MPIHDYGEVDGQMFLEMRLVEGTDLDSVLKRFGPLTPPRAVAIITQIASALDAAHADGVMHRDVKPQNILITRDDFAYLVDFGIASATTDEKLTQLGTAVGTWKYMAPERFSNDEVTYRADIYALACVLHECLTGAPPYRADSAGTLVSSHLMGPIPQPSAIRPGIPKAFDAVVARGMAKKPEDRYASAGDLALAAHEALSDPDQDHAADILRRSQESTLPAPPKPVPPPTMPATAMAPRQPPAPPVTPPGVQPAPKPSYTPPAQPGPAGQRPGPTGQPSWAPNSGPMPASGPTPTPQYYQGGGWGAPPSGGPSPWAQTPRKTNPWPLVAGAAAVVLVLVLGAIGIWIAIRPKPVQPPQPVAEERLSALLLNSSEVNAVMGSSSMQPGKPITSMDSSPVTVSLPDCQGALYTSQDPVYAGTGYTAINGLISSEPGDNYEHWVNQAVVAFPTADKARAFVQTSADKWKNCAGKTVTVTNKAKTYRWTFADVKGSPPTITVIDTQEGAEGWECQRAMSVANNVVVDVNACGYQITNQAGQIAAKIVDKVNKE