Rv1747 Family assigned · medium auto-curated
H37Rv Rv1747 · MTBC0 mtbc0_001860 ·
865 aa · 1985702–1988299 (+) ·
RefSeq NP_216263.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ABC transporter ATP-binding protein/permease |
|---|---|
| MTBC0 PGAP re-annotation | ATP-binding cassette domain-containing protein |
| Revised (this work) | ATP-binding cassette domain-containing protein. Pfam: Yop-YscD_cpl (PF16697.11), FHA (PF00498.32), ABC_tran (PF00005.34), AAA_21 (PF13304.13), ABC2_membrane (PF01061.30). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O65934
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ABC transporter ATP-binding/permease protein Rv1747 |
| EC (curated) |
EC 7.-.-.-
|
| Curated function | Involved in the translocation of an unknown substrate across the membrane. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Required for virulence. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
V Defense mechanisms
|
|---|---|
| eggNOG description | ABC transporter |
| Orthologous group | COG0842 |
| KEGG orthology |
K01990, K21397
|
| KEGG modules |
M00254
|
| Gene Ontology (37) |
GO:0003674, GO:0003824, GO:0005215, GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0006810, GO:0008150 +25 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.082 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 9 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Yop-YscD_cpl | PF16697.11 | 3.2e-10 | 224–301 | Inner membrane component of T3SS, cytoplasmic domain |
FHA | PF00498.32 | 2.9e-17 | 230–293 | FHA domain |
ABC_tran | PF00005.34 | 1.8e-32 | 335–482 | ABC transporter |
AAA_21 | PF13304.13 | 1.2e-07 | 437–514 | AAA domain, putative AbiEii toxin, Type IV TA system |
ABC2_membrane | PF01061.30 | 1.3e-19 | 596–810 | ABC-2 type transporter |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pknF (serine/threonine-protein kinase PknF), high confidence from genomic context alone (score 739 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1248c kgd |
multifunctional 2-oxoglutarate dehydrogenase E1 component /2-oxoglutarate dehydrogenase dihydrolipoyllysine-residue succinyltransferase | 989 | 985 | experimental:984 |
Rv0014c pknB |
serine/threonine-protein kinase PknB | 945 | 801 | experimental:711 textmining:736 |
Rv1746 pknF |
serine/threonine-protein kinase PknF | 952 | 739 ctx | neighborhood:617 textmining:826 |
Rv1364c |
sigma factor regulatory protein | 723 | 712 | coexpression:647 |
Rv1278 hyp |
hypothetical protein | 717 | 700 | database:579 |
Rv1277 hyp |
hypothetical protein | 703 | 692 | database:579 |
Rv0434 hyp |
hypothetical protein | 705 | 676 | database:560 |
Rv3724A cut5a |
Rv3724A, (MTV025.072), len: 80 aa. Probable cut5a,truncated cutinase precursor, similar to N-terminal end of others e.g. Q9KK87 serine ester | 662 | 649 | database:530 |
Rv3724B cut5b |
Rv3724B, (MTV025.072), len: 187 aa. Probable cut5b,truncated cutinase, similar to C-terminal end of others e.g. Q9XB09|RVD2-RV1758 protein ( | 662 | 649 | database:530 |
Rv3483c hyp |
hypothetical protein | 662 | 649 | database:530 |
Rv2301 cut2 |
cutinase | 661 | 648 | database:530 |
Rv3451 cut3 |
cutinase | 660 | 648 | database:530 |
Rv1758 cut1 |
cutinase | 660 | 647 | database:530 |
Rv0908 ctpE |
metal cation transporter ATPase E | 663 | 641 | coexpression:421 |
Rv3132c devS |
two component sensor histidine kinase DevS | 730 | 640 ctx | neighborhood:497 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: ABC transporter ATP-binding protein/permease
- MTBC0 PGAP product: ATP-binding cassette domain-containing protein
- Pfam (hmmscan --cut_ga): Yop-YscD_cpl PF16697.11 (E=3e-10), FHA PF00498.32 (E=3e-17), ABC_tran PF00005.34 (E=2e-32), AAA_21 PF13304.13 (E=1e-07), ABC2_membrane PF01061.30 (E=1e-19)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216263.1)
- Domains: Pfam-A via hmmscan --cut_ga — Yop-YscD_cpl (PF16697.11), FHA (PF00498.32), ABC_tran (PF00005.34), AAA_21 (PF13304.13), ABC2_membrane (PF01061.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0842 - Curated reference: UniProt O65934 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
171 functional partner(s); context anchor
pknF - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001860|Rv1747| MPMSQPAAPPVLTVRYEGSERTFAAGHDVVVGRDLRADVRVAHPLISRAHLLLRFDQGRWVAIDNGSLNGLYLNNRRVPVVDIYDAQRVHIGNPDGPALDFEVGRHRGSAGRPPQTTSIRLPNLSAGAWPTDGPPQTGTLGSGQLQQLPPATTRIPAAPPSGPQPRYPTGGQQLWPPSGPQRAPQIYRPPTAAPPPAGARGGTEAGNLATSMMKILRPGRLTGELPPGAVRIGRANDNDIVIPEVLASRHHATLVPTPGGTEIRDNRSINGTFVNGARVDAALLHDGDVVTIGNIDLVFADGTLARREENLLETRVGGLDVRGVTWTIDGDKTLLDGISLTARPGMLTAVIGPSGAGKSTLARLVAGYTHPTDGTVTFEGHNVHAEYASLRSRIGMVPQDDVVHGQLTVKHALMYAAELRLPPDTTKDDRTQVVARVLEELEMSKHIDTRVDKLSGGQRKRASVALELLTGPSLLILDEPTSGLDPALDRQVMTMLRQLADAGRVVLVVTHSLTYLDVCDQVLLLAPGGKTAFCGPPTQIGPVMGTTNWADIFSTVADDPDAAKARYLARTGPTPPPPPVEQPAELGDPAHTSLFRQFSTIARRQLRLIVSDRGYFVFLALLPFIMGALSMSVPGDVGFGFPNPMGDAPNEPGQILVLLNVGAVFMGTALTIRDLIGERAIFRREQAVGLSTTAYLIAKVCVYTVLAVVQSAIVTVIVLVGKGGPTQGAVALSKPDLELFVDVAVTCVASAMLGLALSAIAKSNEQIMPLLVVAVMSQLVFSGGMIPVTGRVPLDQMSWVTPARWGFAASAATVDLIKLVPGPLTPKDSHWHHTASAWWFDMAMLVALSVIYVGFVRWKIRLKAC