Rv1167c Family assigned · medium auto-curated
H37Rv Rv1167c · MTBC0 mtbc0_001256 ·
201 aa · 1306528–1307133 (-) ·
RefSeq NP_215683.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transcriptional regulator |
|---|---|
| MTBC0 PGAP re-annotation | TetR/AcrR family transcriptional regulator |
| Revised (this work) | TetR/AcrR family transcriptional regulator. Pfam: TetR_N (PF00440.30), TetR_C_11 (PF16859.11). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O50423
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable transcriptional regulatory protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| eggNOG description | Transcriptional regulator |
| Orthologous group | COG1309 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.72 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
TetR_N | PF00440.30 | 1.4e-11 | 24–69 | Bacterial regulatory proteins, tetR family |
TetR_C_11 | PF16859.11 | 1.8e-15 | 85–190 | Tetracyclin repressor-like, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3167c (TetR family transcriptional regulator), high confidence from genomic context alone (score 957 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3167c |
TetR family transcriptional regulator | 957 | 957 ctx | cooccurence:751 coexpression:836 |
Rv3055 |
TetR family transcriptional regulator | 864 | 864 ctx | cooccurence:445 coexpression:765 |
Rv1556 |
HTH-type transcriptional regulator | 841 | 841 | coexpression:785 |
Rv1151c cobB |
NAD-dependent protein deacylase | 841 | 835 | coexpression:835 |
Rv1267c embR |
transcriptional regulator EmbR | 838 | 835 | coexpression:802 |
Rv0653c |
transcriptional regulator | 833 | 833 | coexpression:810 |
Rv1931c |
transcriptional regulator | 831 | 832 | coexpression:806 |
Rv0691c mftR |
mycofactocin biosynthesis transcriptional regulator MftR | 832 | 828 | coexpression:804 |
Rv3830c |
TetR family transcriptional regulator | 831 | 826 | coexpression:802 |
Rv0452 |
transcriptional regulator | 825 | 826 | coexpression:799 |
Rv3082c virS |
HTH-type transcriptional regulator VirS | 826 | 823 | coexpression:797 |
Rv3263 |
DNA methylase | 822 | 822 | coexpression:798 |
Rv1985c lysG |
HTH-type transcriptional regulator | 824 | 821 | coexpression:799 |
Rv3736 |
AraC/XylS family transcriptional regulator | 820 | 817 | coexpression:817 |
Rv3183 higA3 |
transcriptional regulator | 815 | 815 | coexpression:815 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transcriptional regulator
- MTBC0 PGAP product: TetR/AcrR family transcriptional regulator
- Pfam (hmmscan --cut_ga): TetR_N PF00440.30 (E=1e-11), TetR_C_11 PF16859.11 (E=2e-15)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215683.1)
- Domains: Pfam-A via hmmscan --cut_ga — TetR_N (PF00440.30), TetR_C_11 (PF16859.11)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1309 - Curated reference: UniProt O50423 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
74 functional partner(s); context anchor
Rv3167c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001256|Rv1167c| MTVSAPAKANPYRRRGEVLERALYDATLAELESAGYGGLTMEGIAARAQTGKAALYRRWAGKRELVLAAVQYALPPVPEPRADRSARENLLAVFTANCEILAGKTALPSMEIVSQLLHEPELRAIFINSVWAPRLRIVESILQAGVRSGEIDPATLTPMTARIGPALIHQHVLFTGSPPDREQLTRIIDAMILTTGERRES