mshD Resolved · high auto-curated
H37Rv Rv0819 · MTBC0 mtbc0_000868 ·
315 aa · 914803–915750 (+) ·
RefSeq NP_215334.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | mycothiol acetyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | mycothiol synthase |
| Revised (this work) | Mycothiol synthase. Pfam: Acetyltransf_1 (PF00583.32), Acetyltransf_7 (PF13508.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJM7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Mycothiol acetyltransferase |
| EC (curated) |
EC 2.3.1.189
|
| Curated function | Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| Preferred name | mshD |
| eggNOG description | Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol |
| Orthologous group | COG0454 |
| EC number |
EC 2.3.1.189
|
| KEGG orthology |
K15520
|
| Gene Ontology (73) |
GO:0000302, GO:0003674, GO:0003824, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0006790, GO:0006950, GO:0006979, GO:0008080 +61 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.285 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acetyltransf_1 | PF00583.32 | 1.2e-06 | 46–111 | Acetyltransferase (GNAT) family |
Acetyltransf_7 | PF13508.14 | 4.9e-06 | 48–109 | Acetyltransferase (GNAT) domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0818 (transcriptional regulator), high confidence from genomic context alone (score 884 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0818 |
transcriptional regulator | 888 | 884 ctx | neighborhood:882 |
Rv2984 ppk1 |
polyphosphate kinase | 815 | 815 ctx | fusion:783 |
Rv2466c hyp |
hypothetical protein | 798 | 799 ctx | cooccurence:769 |
Rv2130c mshC |
cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase | 989 | 777 ctx | cooccurence:549 database:500 textmining:955 |
Rv1998c hyp |
hypothetical protein | 767 | 768 | coexpression:768 |
Rv1082 mca |
mycothiol S-conjugate amidase | 936 | 767 ctx | cooccurence:763 textmining:739 |
Rv2553c mltG |
membrane protein | 735 | 736 | coexpression:735 |
Rv0360c hyp |
hypothetical protein | 696 | 696 ctx | cooccurence:693 |
Rv1170 mshB |
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase | 984 | 694 ctx | cooccurence:691 textmining:953 |
Rv0820 phoT |
phosphate ABC transporter ATP-binding protein PhoT | 809 | 690 ctx | neighborhood:680 textmining:412 |
Rv2286c hyp |
hypothetical protein | 649 | 650 ctx | cooccurence:646 |
Rv0817c lmeA hyp |
hypothetical protein | 753 | 624 ctx | neighborhood:624 |
Rv0486 mshA |
D-inositol 3-phosphate glycosyltransferase | 972 | 610 ctx | cooccurence:610 textmining:932 |
Rv0816c thiX |
thioredoxin ThiX | 802 | 591 ctx | neighborhood:591 textmining:537 |
Rv2133c hyp |
hypothetical protein | 513 | 514 ctx | cooccurence:507 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: mycothiol acetyltransferase
- MTBC0 PGAP product: mycothiol synthase
- Pfam (hmmscan --cut_ga): Acetyltransf_1 PF00583.32 (E=1e-06), Acetyltransf_7 PF13508.14 (E=5e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215334.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acetyltransf_1 (PF00583.32), Acetyltransf_7 (PF13508.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0454 - Curated reference: UniProt P9WJM7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
48 functional partner(s); context anchor
Rv0818 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000868|Rv0819|mshD MTALDWRSALTADEQRSVRALVTATTAVDGVAPVGEQVLRELGQQRTEHLLVAGSRPGGPIIGYLNLSPPRGAGGAMAELVVHPQSRRRGIGTAMARAALAKTAGRNQFWAHGTLDPARATASALGLVGVRELIQMRRPLRDIPEPTIPDGVVIRTYAGTSDDAELLRVNNAAFAGHPEQGGWTAVQLAERRGEAWFDPDGLILAFGDSPRERPGRLLGFHWTKVHPDHPGLGEVYVLGVDPAAQRRGLGQMLTSIGIVSLARRLGGRKTLDPAVEPAVLLYVESDNVAAVRTYQSLGFTTYSVDTAYALAGTDN