mca Resolved · high auto-curated
H37Rv Rv1082 · MTBC0 mtbc0_001162 ·
288 aa · 1214789–1215655 (+) ·
RefSeq NP_215598.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | mycothiol S-conjugate amidase |
|---|---|
| MTBC0 PGAP re-annotation | mycothiol conjugate amidase Mca |
| Revised (this work) | Mycothiol conjugate amidase Mca. Pfam: PIG-L (PF02585.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJN1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Mycothiol S-conjugate amidase |
| EC (curated) |
EC 3.5.1.115
|
| Curated function | A mycothiol (MSH, N-acetyl-cysteinyl-glucosaminyl-inositol) S-conjugate amidase, it recycles conjugated MSH to the N-acetyl cysteine conjugate and the MSH precursor. Involved in MSH-dependent detoxification of a number of alkylating agents and antibiotics. Activity is specific for the mycothiol moiety. Has a low but measurable deacetylation activity on GlcNAc-Ins (N-acetyl-glucosaminyl-inositol), and thus can also directly contribute to the production of MSH. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mca |
| eggNOG description | A mycothiol (MSH, N-acetylcysteinyl-glucosaminyl- inositol) S-conjugate amidase, it recycles conjugated MSH to the N-acetyl cysteine conjugate (AcCys S-conjugate, a mercapturic acid) and the MSH precursor. Involved in MSH-dependent detoxification of a number of alkylating agents and antibiotics |
| Orthologous group | COG2120 |
| EC number |
EC 3.5.1.115
|
| KEGG orthology |
K18455
|
| Gene Ontology (23) |
GO:0003674, GO:0005488, GO:0006790, GO:0008150, GO:0008152, GO:0008270, GO:0009636, GO:0009987, GO:0010126, GO:0010127, GO:0016137, GO:0042221 +11 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.633 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PIG-L | PF02585.23 | 1.8e-29 | 7–152 | GlcNAc-PI de-N-acetylase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1081c (membrane protein), high confidence from genomic context alone (score 780 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1083 hyp |
hypothetical protein | 888 | 888 ctx | neighborhood:881 |
Rv1084 hyp |
hypothetical protein | 885 | 885 ctx | neighborhood:882 |
Rv2466c hyp |
hypothetical protein | 789 | 789 ctx | cooccurence:773 |
Rv1081c |
membrane protein | 780 | 780 ctx | neighborhood:780 |
Rv0819 mshD |
mycothiol acetyltransferase | 936 | 767 ctx | cooccurence:763 textmining:739 |
Rv0360c hyp |
hypothetical protein | 741 | 741 ctx | cooccurence:741 |
Rv2286c hyp |
hypothetical protein | 701 | 701 ctx | cooccurence:700 |
Rv0486 mshA |
D-inositol 3-phosphate glycosyltransferase | 919 | 692 ctx | cooccurence:668 textmining:748 |
Rv2130c mshC |
cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase | 941 | 594 ctx | cooccurence:563 textmining:862 |
Rv2133c hyp |
hypothetical protein | 571 | 571 ctx | cooccurence:569 |
Rv1080c greA |
transcription elongation factor GreA | 543 | 543 ctx | neighborhood:468 |
Rv2134c hyp |
hypothetical protein | 514 | 515 ctx | cooccurence:509 |
Rv0498 hyp |
hypothetical protein | 487 | 487 ctx | cooccurence:470 |
Rv1331 clpS |
ATP-dependent Clp protease adapter protein ClpS | 486 | 487 ctx | cooccurence:485 |
Rv2111c pup |
ubiquitin-like protein Pup | 433 | 433 ctx | cooccurence:425 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: mycothiol S-conjugate amidase
- MTBC0 PGAP product: mycothiol conjugate amidase Mca
- Pfam (hmmscan --cut_ga): PIG-L PF02585.23 (E=2e-29)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215598.1)
- Domains: Pfam-A via hmmscan --cut_ga — PIG-L (PF02585.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2120 - Curated reference: UniProt P9WJN1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
32 functional partner(s); context anchor
Rv1081c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001162|Rv1082|mca MSELRLMAVHAHPDDESSKGAATLARYADEGHRVLVVTLTGGERGEILNPAMDLPDVHGRIAEIRRDEMTKAAEILGVEHTWLGFVDSGLPKGDLPPPLPDDCFARVPLEVSTEALVRVVREFRPHVMTTYDENGGYPHPDHIRCHQVSVAAYEAAGDFCRFPDAGEPWTVSKLYYVHGFLRERMQMLQDEFARHGQRGPFEQWLAYWDPDHDFLTSRVTTRVECSKYFSQRDDALRAHATQIDPNAEFFAAPLAWQERLWPTEEFELARSRIPARPPETELFAGIEP