Rv0691A Resolved · high auto-curated
H37Rv Rv0691A · MTBC0 - ·
29 aa · 791757–791846 (+) ·
RefSeq YP_007409348.2
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | mycofactocin precursor |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Mycofactocin precursor. Pfam: MftA (PF23709.2). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WJ81
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Mycofactocin precursor peptide |
| Curated function | Precursor peptide that leads to mycofactocin (MFT) after extensive post-translational modifications by enzymes encoded by adjacent genes. Mycofactocin acts as a redox cofactor of nicotinamide-dependent oxidoreductases encoded in the same locus. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Orthologous group | 2B3XY |
|---|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MftA | PF23709.2 | 5.3e-16 | 1–29 | Mycofactocin precursor peptide |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mftB (mycofactocin system protein MftB), high confidence from genomic context alone (score 882 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0692 mftB |
mycofactocin system protein MftB | 984 | 882 ctx | neighborhood:882 textmining:870 |
Rv0694 mftD |
mycofactocin system heme/flavin oxidoreductase MftD | 976 | 882 ctx | neighborhood:882 textmining:810 |
Rv0693 mftC |
mycofactocin radical SAM maturase MftC | 957 | 882 ctx | neighborhood:882 textmining:655 |
Rv0691c mftR |
mycofactocin biosynthesis transcriptional regulator MftR | 573 | 573 ctx | neighborhood:573 |
Rv0690c hyp |
hypothetical protein | 573 | 573 ctx | neighborhood:573 |
Rv0695 mftE |
mycofactocin system creatinine amidohydrolase family protein MftE | 934 | 514 ctx | neighborhood:514 textmining:870 |
Rv0696 mftF |
mycofactocin biosynthesis glycosyltransferase MftF | 925 | 448 ctx | neighborhood:448 textmining:870 |
Rv0697 mftG |
dehydrogenase | 423 | 423 ctx | neighborhood:423 |
Rv1872c lldD2 |
L-lactate dehydrogenase | 497 | 72 | textmining:481 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): mycofactocin precursor
- Pfam (hmmscan --cut_ga): MftA PF23709.2 (E=5e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_007409348.2)
- Domains: Pfam-A via hmmscan --cut_ga — MftA (PF23709.2)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2B3XY - Curated reference: UniProt P9WJ81 (SwissProt, reviewed; Inferred from homology)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
9 functional partner(s); context anchor
mftB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0691A| MDYETDTDTELVTETLVEEVSIDGMCGVY