eno Resolved · high auto-curated
H37Rv Rv1023 · MTBC0 mtbc0_001099 ·
429 aa · 1151901–1153190 (+) ·
RefSeq NP_215539.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | enolase |
|---|---|
| MTBC0 PGAP re-annotation | phosphopyruvate hydratase |
| Revised (this work) | Phosphopyruvate hydratase. Pfam: Enolase_N (PF03952.22), Enolase_C (PF00113.29), MR_MLE_C (PF13378.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WNL1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Enolase |
| EC (curated) |
EC 4.2.1.11
|
| Curated function | Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis..; FUNCTION: 'Moonlights' as a plasminogen receptor. Protein purifed from E.coli binds immobilized host (human) plasminogen with a dissociation constant of 360 nM; 0.1 M lysine prevents plasminogen binding. Protein purifed from M.tuberculosis H37Ra binds immobilized host plasminogen with a dissociation constant of 398 nM, in the same paper protein purified from E.coli binds with a dissociation constant of 888 nM. Overexpression (in. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
F Nucleotide transport and metabolism
|
|---|---|
| Preferred name | eno |
| eggNOG description | Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis |
| Orthologous group | COG4948 |
| EC number |
EC 4.2.1.11
|
| KEGG orthology |
K01689
|
| KEGG pathways |
map00010, map00680, map01100, map01110, map01120, map01130, map01200, map01230, map03018, map04066
|
| KEGG modules |
M00001, M00002, M00003, M00346, M00394
|
| Gene Ontology (10) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0008150, GO:0016020, GO:0030312, GO:0040007, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.606 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Enolase_N | PF03952.22 | 1.0e-56 | 4–133 | Enolase, N-terminal domain |
Enolase_C | PF00113.29 | 8.8e-125 | 139–418 | Enolase, C-terminal TIM barrel domain |
MR_MLE_C | PF13378.13 | 4.2e-08 | 263–380 | Enolase C-terminal domain-like |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pgk (phosphoglycerate kinase), high confidence from genomic context alone (score 985 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1617 pykA |
pyruvate kinase | 998 | 987 | coexpression:819 database:900 textmining:857 |
Rv1437 pgk |
phosphoglycerate kinase | 995 | 985 ctx | cooccurence:685 coexpression:858 experimental:665 textmining:733 |
Rv0489 gpm1 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 987 | 971 | coexpression:665 database:900 textmining:587 |
Rv0946c pgi |
glucose-6-phosphate isomerase | 993 | 966 | coexpression:799 database:800 textmining:819 |
Rv1436 gap |
glyceraldehyde 3-phosphate dehydrogenase | 990 | 966 ctx | cooccurence:591 coexpression:842 experimental:474 textmining:745 |
Rv0363c fba |
fructose-bisphosphate aldolase | 986 | 964 | coexpression:802 database:800 textmining:636 |
Rv1449c tkt |
transketolase | 967 | 945 | coexpression:642 database:800 textmining:434 |
Rv1438 tpi |
triosephosphate isomerase | 985 | 941 | coexpression:858 experimental:476 textmining:762 |
Rv0702 rplD |
50S ribosomal protein L4 | 943 | 937 | coexpression:681 experimental:794 |
Rv1127c ppdK |
pyruvate, phosphate dikinase PpdK | 917 | 906 | database:900 |
Rv1024 |
membrane protein | 903 | 904 ctx | neighborhood:882 |
Rv0211 pckA |
phosphoenolpyruvate carboxykinase | 918 | 900 | database:900 |
Rv1448c tal |
transaldolase | 977 | 893 | coexpression:429 database:800 textmining:797 |
Rv1389 gmk |
guanylate kinase | 902 | 889 | experimental:781 |
Rv0904c accD3 |
acetyl-CoAcarboxylase carboxyl transferase subunit beta | 883 | 880 | experimental:863 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: enolase
- MTBC0 PGAP product: phosphopyruvate hydratase
- Pfam (hmmscan --cut_ga): Enolase_N PF03952.22 (E=1e-56), Enolase_C PF00113.29 (E=9e-125), MR_MLE_C PF13378.13 (E=4e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215539.1)
- Domains: Pfam-A via hmmscan --cut_ga — Enolase_N (PF03952.22), Enolase_C (PF00113.29), MR_MLE_C (PF13378.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4948 - Curated reference: UniProt P9WNL1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
218 functional partner(s); context anchor
pgk - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001099|Rv1023|eno MPIIEQVGAREILDSRGNPTVEVEVALIDGTFARAAVPSGASTGEHEAVELRDGGDRYGGKGVQKAVQAVLDEIGPAVIGLNADDQRLVDQALVDLDGTPDKSRLGGNAILGVSLAVAKAAADSAELPLFRYVGGPNAHILPVPMMNILNGGAHADTAVDIQEFMVAPIGAPSFVEALRWGAEVYHALKSVLKKEGLSTGLGDEGGFAPDVAGTTAALDLISRAIESAGLRPGADVALALDAAATEFFTDGTGYVFEGTTRTADQMTEFYAGLLGAYPLVSIEDPLSEDDWDGWAALTASIGDRVQIVGDDIFVTNPERLEEGIERGVANALLVKVNQIGTLTETLDAVTLAHHGGYRTMISHRSGETEDTMIADLAVAIGSGQIKTGAPARSERVAKYNQLLRIEEALGDAARYAGDLAFPRFACETK