Rv0295c Family assigned · medium auto-curated
H37Rv Rv0295c · MTBC0 mtbc0_000314 ·
267 aa · 362239–363042 (-) ·
RefSeq NP_214809.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | Stf0 family sulfotransferase |
| Revised (this work) | Stf0 family sulfotransferase. Pfam: Sulphotransf (PF09037.17). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53699
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Trehalose 2-sulfotransferase |
| EC (curated) |
EC 2.8.2.37
|
| Curated function | Catalyzes the sulfuryl group transfer from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid and the most abundant sulfated metabolite found in Mycobacterium tuberculosis, that is a potential virulence factor thought to mediate host-pathogen interactions. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | stf0 |
| eggNOG description | carbohydrate metabolic process |
| Orthologous group | COG4424 |
| EC number |
EC 2.8.2.37
|
| KEGG orthology |
K21014
|
| Gene Ontology (81) |
GO:0000166, GO:0003674, GO:0003824, GO:0005488, GO:0005515, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0005975, GO:0005984, GO:0005991 +69 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 3 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.10% of strains (148) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Sulphotransf | PF09037.17 | 3.3e-108 | 8–259 | Stf0 sulphotransferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0296c (sulfatase), high confidence from genomic context alone (score 968 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0296c |
sulfatase | 968 | 968 ctx | neighborhood:882 cooccurence:741 |
Rv1182 papA3 |
acyltransferase papA3 | 793 | 651 ctx | cooccurence:649 textmining:433 |
Rv1274 lprB |
lipoprotein LprB | 635 | 635 ctx | cooccurence:633 |
Rv0210 hyp |
hypothetical protein | 622 | 622 ctx | cooccurence:620 |
Rv3824c papA1 |
acyltransferase | 773 | 616 ctx | cooccurence:616 textmining:434 |
Rv0180c |
transmembrane protein | 611 | 611 ctx | cooccurence:609 |
Rv3820c papA2 |
trehalose-2-sulfate acyltransferase | 909 | 562 ctx | cooccurence:559 textmining:803 |
Rv0713 |
transmembrane protein | 549 | 550 ctx | cooccurence:543 |
Rv0306 bluB |
oxidoreductase | 545 | 545 ctx | neighborhood:544 |
Rv1275 lprC |
lipoprotein LprC | 539 | 540 ctx | cooccurence:535 |
Rv0197 |
oxidoreductase | 534 | 534 ctx | cooccurence:534 |
Rv0297 PE_PGRS5 |
PE-PGRS family protein PE_PGRS5 | 524 | 524 ctx | neighborhood:524 |
Rv1303 hyp |
hypothetical protein | 523 | 523 ctx | cooccurence:523 |
Rv2687c |
antibiotic ABC transporter permease | 516 | 516 ctx | cooccurence:516 |
Rv0383c ttfA hyp |
hypothetical protein | 514 | 514 ctx | cooccurence:514 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: Stf0 family sulfotransferase
- Pfam (hmmscan --cut_ga): Sulphotransf PF09037.17 (E=3e-108)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214809.1)
- Domains: Pfam-A via hmmscan --cut_ga — Sulphotransf (PF09037.17)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4424 - Curated reference: UniProt O53699 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
38 functional partner(s); context anchor
Rv0296c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000314|Rv0295c| MSRAVRPYLVLATQRSGSTLLVESLRATGCAGEPQEFFQYLPSTGMAPQPREWFAGVDDDTILQLLDPLDPGTPDTATPVAWREHVRTSGRTPNGVWGGKLMWNQTALLQQRAAQLPDRSGDGLRAAIRDVIGNEPVFVHVHRPDVVSQAVSFWRAVQTQVWRGHPDPKRDSQAVYHAGAIAHIIRNLRDQENGWRAWFAEEGIDPIDIAYPVLWRNLTAIVASVLDAIGQDPKLAPAPMLERQANQRSDEWVDRYRAEAPRLGLPT