Rv0296c Resolved · high auto-curated
H37Rv Rv0296c · MTBC0 - ·
465 aa · 359758–361155 (-) ·
RefSeq YP_177712.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | sulfatase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Sulfatase. Pfam: Sulfatase (PF00884.29), Phosphodiest (PF01663.28), SGSH_C (PF16347.11). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
Q6MX51
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable sulfatase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | atsG |
| eggNOG description | sulfatase |
| Orthologous group | COG3119 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.205 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Sulfatase | PF00884.29 | 5.3e-38 | 12–286 | Sulfatase |
Phosphodiest | PF01663.28 | 5.6e-07 | 34–235 | Type I phosphodiesterase / nucleotide pyrophosphatase |
SGSH_C | PF16347.11 | 1.4e-08 | 242–363 | N-sulphoglucosamine sulphohydrolase, C-terminal |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: atsA (arylsulfatase AtsA), medium confidence from genomic context alone (score 587 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0295c stf0 hyp |
hypothetical protein | 968 | 968 ctx | neighborhood:882 cooccurence:741 |
Rv0712 hyp |
hypothetical protein | 844 | 845 ctx | cooccurence:706 experimental:430 |
Rv1213 glgC |
glucose-1-phosphate adenylyltransferase | 606 | 602 | coexpression:602 |
Rv0711 atsA |
arylsulfatase AtsA | 587 | 587 ctx | cooccurence:587 |
Rv0303 |
dehydrogenase/reductase | 581 | 574 | coexpression:401 |
Rv0342 iniA |
isoniazid inductible protein IniA | 573 | 573 | database:517 |
Rv3077 |
hydrolase | 559 | 559 ctx | cooccurence:552 |
Rv0306 bluB |
oxidoreductase | 554 | 554 ctx | neighborhood:544 |
Rv0307c hyp |
hypothetical protein | 546 | 547 ctx | neighborhood:544 |
Rv2874 dipZ |
integral membrane C-type cytochrome biogenesis protein DipZ | 555 | 538 | |
Rv0297 PE_PGRS5 |
PE-PGRS family protein PE_PGRS5 | 524 | 524 ctx | neighborhood:524 |
Rv0663 atsD |
arylsulfatase AtsD | 517 | 518 ctx | cooccurence:515 |
Rv1527c pks5 |
polyketide synthase | 529 | 499 | coexpression:408 |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 527 | 497 | coexpression:405 |
Rv2048c pks12 |
polyketide synthase | 525 | 495 | coexpression:403 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): sulfatase
- Pfam (hmmscan --cut_ga): Sulfatase PF00884.29 (E=5e-38), Phosphodiest PF01663.28 (E=6e-07), SGSH_C PF16347.11 (E=1e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177712.1)
- Domains: Pfam-A via hmmscan --cut_ga — Sulfatase (PF00884.29), Phosphodiest (PF01663.28), SGSH_C (PF16347.11)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3119 - Curated reference: UniProt Q6MX51 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
73 functional partner(s); context anchor
atsA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0296c| MTSERATGQRENLLIVHWHDLGRYLGVYHHPDVYSPRLDRLAAEGILFTRAHATAPLCTPSRGSLFTGRYPQSNGLVGLAHHGWEYRTGVQTLPQLLSESGWYSALFGMQHETSYPKRLGFDEFDVSNSYCEYVVAKAQDWLHNRVPALDGQRFLLTAGFFETHRPYPHERYRPADSAAVELPDYLPDTPEVRQDVAEFYGSIATADEAVGRLLDTLADTGLDASTWVVFVTDHGPAFPRAKSTLYDAGTGIALIIRPPTRRAMAPRVYDELFSGVDLVPTLLDLLRLEVPADVEGVSHAPALLAPDTENAAVRDHVYTAKTYHDSFDPIRAIRTKEYSYIENYAPRPLLDLPWDIQESPAGMAVAPLVKAPRPQRELYDLRADPTETNNLLAGDDSTQGVAAIAADLAVRLHDWRQRTADVIPSDFAGSRIAERYTETYLRIHRKTPTGRSAIAADRGIDEHCS