Rv0218 Resolved · high auto-curated
H37Rv Rv0218 · MTBC0 mtbc0_000232 ·
442 aa · 261305–262633 (+) ·
RefSeq NP_214732.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | molybdopterin-dependent oxidoreductase |
| Revised (this work) | Molybdopterin-dependent oxidoreductase. Pfam: Oxidored_molyb (PF00174.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P96400
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Probable conserved transmembrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Oxidoreductase molybdopterin binding |
| Orthologous group | COG2041 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 2.691 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Oxidored_molyb | PF00174.26 | 5.2e-20 | 310–441 | Oxidoreductase molybdopterin binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0219 (transmembrane protein), high confidence from genomic context alone (score 972 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0219 |
transmembrane protein | 972 | 972 ctx | neighborhood:881 cooccurence:771 |
Rv0540 hyp |
hypothetical protein | 885 | 886 ctx | neighborhood:521 cooccurence:771 |
Rv0539 |
dolichyl-phosphate sugar synthase | 877 | 877 ctx | neighborhood:521 cooccurence:753 |
Rv0220 lipC |
esterase LipC | 868 | 869 ctx | neighborhood:867 |
Rv0536 galE3 |
UDP-glucose 4-epimerase GalE | 804 | 805 ctx | cooccurence:769 |
Rv0541c |
integral membrane protein | 802 | 802 ctx | cooccurence:767 |
Rv2194 qcrC |
ubiquinol-cytochrome C reductase cytochrome subunit C | 845 | 798 | experimental:790 |
Rv1999c |
transporter | 788 | 781 | coexpression:774 |
Rv0222 echA1 |
enoyl-CoA hydratase EchA1 | 765 | 764 ctx | neighborhood:758 |
Rv0221 |
diacyglycerol O-acyltransferase | 662 | 662 ctx | neighborhood:659 |
Rv0217c lipW |
esterase LipW | 641 | 641 ctx | neighborhood:636 |
Rv0650 |
sugar kinase | 431 | 432 | coexpression:432 |
Rv2428 ahpC |
alkyl hydroperoxide reductase subunit AhpC | 482 | 430 | coexpression:430 |
Rv1937 |
oxygenase | 501 | 370 | |
Rv0535 pnp |
5'-methylthioadenosine phosphorylase | 400 | 369 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: molybdopterin-dependent oxidoreductase
- Pfam (hmmscan --cut_ga): Oxidored_molyb PF00174.26 (E=5e-20)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214732.1)
- Domains: Pfam-A via hmmscan --cut_ga — Oxidored_molyb (PF00174.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2041 - Curated reference: UniProt P96400 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
28 functional partner(s); context anchor
Rv0219 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000232|Rv0218| MSDPARGAEAEDAYGFPAGLWRWLQRHPPPALHRLTRFRSPLRGPWLTSVFGLVLLVALPFVIITGLLSYIAYAPQLGQAIPGDVGWLRLPAFTWPTRPSWLYRLTQGLHVGLGLVIIPVVLAKLWSVIPRLFVWPPARSIAQVLERLSVLMLVGGILFQIVTGVLNIQYDYIFGFSFYTGHYFGAWVFIAGFLLHIVVKIPHMVTGLRSIPMREVLGTNVADTRAQPCDPDGLVSVNPGEATLSRRGALGLVGAGVLLIGVLTVGQTLGGFTRKAALLLPRGRVVSPGDFPVNKTAAAAGITAEAIGPDWRLVLRGGPAEVVLDRATLAGLPQRTARLPLACVEGWSAVRTWSGVPLAELALLAGVPAARSARVTSLQRGGAFGEAKLAANQIADPDALLALRVDGADLSLDHGYPARIIVPALPGVHNTKWVAGIEFHKR