Rv0228 Resolved · high auto-curated
H37Rv Rv0228 · MTBC0 mtbc0_000242 ·
407 aa · 273436–274659 (+) ·
RefSeq NP_214742.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | acyltransferase |
| Revised (this work) | Acyltransferase. Pfam: Acyl_transf_3 (PF01757.29). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P96410
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable integral membrane acyltransferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Acyltransferase |
| Orthologous group | COG1835 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.771 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acyl_transf_3 | PF01757.29 | 6.4e-29 | 38–370 | Acyltransferase domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0226c (transmembrane protein), high confidence from genomic context alone (score 773 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0226c |
transmembrane protein | 831 | 773 ctx | neighborhood:582 cooccurence:466 |
Rv0227c |
membrane protein | 748 | 744 ctx | neighborhood:582 cooccurence:409 |
Rv3802c |
membrane protein | 608 | 588 ctx | cooccurence:571 |
Rv0224c |
methyltransferase | 571 | 571 ctx | cooccurence:567 |
Rv3803c fbpD |
MPT51/MPB51 antigen | 568 | 568 ctx | cooccurence:565 |
Rv0111 |
acyltransferase | 540 | 541 ctx | cooccurence:535 |
Rv2673 aftC |
alpha-(1->3)-arabinofuranosyltransferase | 521 | 521 ctx | cooccurence:514 |
Rv0225 hyp |
hypothetical protein | 531 | 507 ctx | cooccurence:476 |
Rv0129c fbpC |
diacylglycerol acyltransferase/mycolyltransferase Ag85C | 478 | 473 ctx | cooccurence:470 |
Rv1476 |
membrane protein | 471 | 472 ctx | cooccurence:471 |
Rv1489A hyp |
hypothetical protein | 462 | 463 ctx | cooccurence:462 |
Rv3035 hyp |
hypothetical protein | 448 | 449 ctx | cooccurence:440 |
Rv1354c hyp |
hypothetical protein | 449 | 448 | coexpression:416 |
Rv2721c hyp |
hypothetical protein | 446 | 447 ctx | cooccurence:432 |
Rv3635 |
transmembrane protein | 457 | 433 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acyltransferase
- MTBC0 PGAP product: acyltransferase
- Pfam (hmmscan --cut_ga): Acyl_transf_3 PF01757.29 (E=6e-29)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214742.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acyl_transf_3 (PF01757.29)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1835 - Curated reference: UniProt P96410 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
33 functional partner(s); context anchor
Rv0226c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000242|Rv0228| MGPADESGAPIRPQTPHRHTVLVTNGQVVGGTRGFLPAVEGMRACAAVGVVVTHVAFQTGHSSGVGGRLFGRFDLAVAVFFAVSGFLLWRGHAAAARDLRSHPRTGPYLRSRVARIMPAYVVAVVVILSLLPDADHASLTVWLANLTLTQIYVPLTLTGGLTQMWSLSVEVAFYAALPVLALLGRRIPVGARVPAIAALAALSWAWGWLPLDAGSGINPLTWPPAFFSWFAAGMLLAEWAYSPVGLPHRWARRRVAMAVTALLGYLVAASPLAGPEGLVPGTAAQFAVKTAMGSLVAFALVAPLVLDRPDTSHRLLGSPAMVTLGRWSYGLFIWHLAALAMVFPVIGAFPFTGRMPTVLVLTLIFGFAIAAVSYALVESPCREALRRWERRNEPISVGELQADAIAP