atsB Resolved · high auto-curated
H37Rv Rv3299c · MTBC0 mtbc0_003507 ·
970 aa · 3705131–3708043 (-) ·
RefSeq NP_217816.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | arylsulfatase AtsB |
|---|---|
| MTBC0 PGAP re-annotation | arylsulfatase AtsB |
| Revised (this work) | Arylsulfatase AtsB. Pfam: DUF1269 (PF06897.18), Sulfatase (PF00884.29). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O65931
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable arylsulfatase AtsB |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | atsB |
| eggNOG description | thought to play an important role in the mineralization of sulfates catalytic activity a phenol sulfate H2O a phenol sulfate |
| Orthologous group | COG3119 |
| EC number |
EC 3.1.6.1
|
| KEGG orthology |
K01130
|
| KEGG pathways |
map00140, map00600
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.502 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 15 synonymous, 22 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.45% of strains (658) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DUF1269 | PF06897.18 | 1.6e-09 | 66–159 | Protein of unknown function (DUF1269) |
Sulfatase | PF00884.29 | 2.5e-71 | 212–634 | Sulfatase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: phoY1 (phosphate transport system transcriptional regulator PhoY), high confidence from genomic context alone (score 777 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3300c hyp |
hypothetical protein | 948 | 948 ctx | neighborhood:756 coexpression:796 |
Rv0712 hyp |
hypothetical protein | 846 | 846 ctx | cooccurence:721 experimental:430 |
Rv3301c phoY1 |
phosphate transport system transcriptional regulator PhoY | 777 | 777 ctx | neighborhood:714 |
Rv3298c lpqC |
esterase LpqC | 762 | 762 ctx | neighborhood:703 |
Rv3493c |
Mce associated protein | 730 | 730 | coexpression:730 |
Rv3302c glpD2 |
glycerol-3-phosphate dehydrogenase | 611 | 611 ctx | neighborhood:541 |
Rv1213 glgC |
glucose-1-phosphate adenylyltransferase | 610 | 606 | coexpression:606 |
Rv3492c |
Mce associated protein | 555 | 539 | |
Rv0178 |
Mce associated membrane protein | 543 | 526 | |
Rv0342 iniA |
isoniazid inductible protein IniA | 517 | 518 | database:517 |
Rv1663 pks17 |
polyketide synthase | 531 | 513 | coexpression:431 |
Rv3303c lpdA |
NAD(P)H quinone reductase LpdA | 509 | 509 ctx | neighborhood:496 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 531 | 501 | coexpression:410 |
Rv2857c |
3-oxoacyl-ACP reductase | 506 | 498 | coexpression:425 |
Rv1527c pks5 |
polyketide synthase | 527 | 497 | coexpression:405 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: arylsulfatase AtsB
- MTBC0 PGAP product: arylsulfatase AtsB
- Pfam (hmmscan --cut_ga): DUF1269 PF06897.18 (E=2e-09), Sulfatase PF00884.29 (E=2e-71)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217816.1)
- Domains: Pfam-A via hmmscan --cut_ga — DUF1269 (PF06897.18), Sulfatase (PF00884.29)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3119 - Curated reference: UniProt O65931 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
66 functional partner(s); context anchor
phoY1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003507|Rv3299c|atsB MMSEDNALVLVAGYQDLDSARHDFQTLVDAAKDKSIPLQGAVLIGKDAEGSPVLVDTGNRLGRRGAAWGAGVGLAIGLFSPALLASAALGAATGALAGTFAHHRIKTGLADKIGQALAAGRAVVIAVTEAQGRLEAGQALASSPMKSVAELSRSTLRSLGAALREAMGKFNPDRTRLPLPQRRFGGVVGRTMAESVGDWSIVPGPFPPDDAPNVLIVLIDDAGFGGPDTFGGAIRTPTLSRLAQNGLIYNRFHVTAVCSPTRAALLTGRNHHRVGFGSVCEFPGPYPGYSAVRPRSCAALPRILRDNGYVTGAFGKWHLTPDNVQGAAGPFDNWPLGWGFDHFWGFPSGAAGQYDPIISQDNSVIGIPEGSGEDGRPYYFPDDLTDKAIEWLHTVRAQNATKPWMLYYATGATHAPHHVFKEWADKYRGEFDDGWDVYRQKTFERQKRLGIIPPDAELTERPDLFPAWDSMSEAQKRLFARQMEVFAGFSENADWNVGRLLDAIEDLGESDNTLVFYIWGDNGASMEGTNTGSFNEMTFLNGLDLDAERQLELIEQYGGIAALGDEFTAPHFASAWAHASNTPLQWGKQMASHLGGTRDPLVVAWPARIRPDGRVRSQFTHCIDIAPTVLAAIGLPEPTHVDGFEQEPMDGTSFVRTFDDAEAEDRHTVQYFENFGSRAIYKDGWWACARLDKAPWDLSPETMRRFAPGTYDPDQDVWELYYLPDDFSQAKNLAAEHPDKVAELTQLWWQEAERNRVLPLLGGLAVMFGDLPPLPTTARFSFKGDVQNIQRGMVPRICGRSYAIEARLHIPDGGAQGVIVANADFMGGFALWVDEQRHLHHTYSFLGVETYRQVSSEPLPTGDVTVRMLFDSHQPVAASGGRVTLWADDRLIGEGELPQTVPLAFTSYAGMDIGRDNGLVVDRGYEDKAPYAFTGTVTEVIFDLKPVHPEAARALHEHASVQAVGQGAAG