glgC Resolved · high auto-curated
H37Rv Rv1213 · MTBC0 - ·
404 aa · 1355836–1357050 (+) ·
RefSeq NP_215729.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | glucose-1-phosphate adenylyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Glucose-1-phosphate adenylyltransferase. Pfam: NTP_transferase (PF00483.30), Hexapep_GlmU (PF24894.2). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WN43
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Glucose-1-phosphate adenylyltransferase |
| EC (curated) |
EC 2.7.7.27
|
| Curated function | Involved in the biosynthesis of ADP-glucose building block required in the biosynthesis of maltose-1-phosphate (M1P) and in the elongation reactions to produce linear alpha-1,4-glucans. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | glgC |
| eggNOG description | Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans |
| Orthologous group | COG0448 |
| EC number |
EC 2.7.7.27
|
| KEGG orthology |
K00975
|
| KEGG pathways |
map00500, map00520, map01100, map01110, map02026
|
| KEGG modules |
M00565
|
| Gene Ontology (32) |
GO:0000271, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005975, GO:0005976, GO:0006073, GO:0008150, GO:0008152, GO:0009058 +20 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.247 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
NTP_transferase | PF00483.30 | 6.6e-71 | 9–276 | Nucleotidyl transferase |
Hexapep_GlmU | PF24894.2 | 1.1e-28 | 301–398 | GlmU-like, C-terminal hexapeptide |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: glgA (capsular glucan synthase), high confidence from genomic context alone (score 987 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1212c glgA |
capsular glucan synthase | 999 | 987 ctx | neighborhood:765 cooccurence:465 database:900 textmining:965 |
Rv1328 glgP |
glycogen phosphorylase | 999 | 982 | coexpression:765 database:900 textmining:948 |
Rv1326c glgB |
1,4-alpha-glucan branching protein | 997 | 944 ctx | cooccurence:763 coexpression:734 textmining:959 |
Rv1781c malQ |
4-alpha-glucanotransferase | 993 | 938 | database:900 textmining:893 |
Rv3068c pgmA |
phosphoglucomutase PgmA | 952 | 913 | database:900 textmining:484 |
Rv0993 galU |
UTP--glucose-1-phosphate uridylyltransferase | 984 | 901 | database:900 textmining:855 |
Rv1564c treX |
maltooligosyl trehalose synthase | 978 | 843 ctx | cooccurence:489 coexpression:668 textmining:866 |
Rv1658 argG |
argininosuccinate synthase | 860 | 818 | coexpression:803 |
Rv3032 |
glycogen synthase | 978 | 809 | database:800 textmining:891 |
Rv3490 otsA |
trehalose-phosphate synthase | 978 | 802 | database:800 textmining:898 |
Rv1562c treZ |
malto-oligosyltrehalose trehalohydrolase | 980 | 798 | coexpression:732 textmining:909 |
Rv1652 argC |
N-acetyl-gamma-glutamyl-phoshate reductase | 833 | 783 | coexpression:765 |
Rv1654 argB |
acetylglutamate kinase | 779 | 712 | coexpression:688 |
Rv3417c groEL1 |
chaperonin GroEL | 649 | 611 | database:596 |
Rv0440 groEL2 |
molecular chaperone GroEL | 691 | 608 | database:596 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): glucose-1-phosphate adenylyltransferase
- Pfam (hmmscan --cut_ga): NTP_transferase PF00483.30 (E=7e-71), Hexapep_GlmU PF24894.2 (E=1e-28)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215729.1)
- Domains: Pfam-A via hmmscan --cut_ga — NTP_transferase (PF00483.30), Hexapep_GlmU (PF24894.2)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0448 - Curated reference: UniProt P9WN43 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
86 functional partner(s); context anchor
glgA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1213|glgC MREVPHVLGIVLAGGEGKRLYPLTADRAKPAVPFGGAYRLIDFVLSNLVNARYLRICVLTQYKSHSLDRHISQNWRLSGLAGEYITPVPAQQRLGPRWYTGSADAIYQSLNLIYDEDPDYIVVFGADHVYRMDPEQMVRFHIDSGAGATVAGIRVPRENATAFGCIDADDSGRIRSFVEKPLEPPGTPDDPDTTFVSMGNYIFTTKVLIDAIRADADDDHSDHDMGGDIVPRLVADGMAAVYDFSDNEVPGATDRDRAYWRDVGTLDAFYDAHMDLVSVHPVFNLYNKRWPIRGESENLAPAKFVNGGSAQESVVGAGSIISAASVRNSVLSSNVVVDDGAIVEGSVIMPGTRVGRGAVVRHAILDKNVVVGPGEMVGVDLEKDRERFAISAGGVVAVGKGVWI