Rv2206 Still unknown · low auto-curated
H37Rv Rv2206 · MTBC0 mtbc0_002342 ·
236 aa · 2496779–2497489 (+) ·
RefSeq NP_216722.2
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | DUF3043 domain-containing protein |
| Revised (this work) | Conserved hypothetical protein; DUF domain(s) DUF3043. Function unknown. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLI5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv2206 |
UniProt still lists this protein as Uncharacterized protein Rv2206; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Protein of unknown function (DUF3043) |
| Orthologous group | 2AN86 |
| Gene Ontology (25) |
GO:0008150, GO:0009605, GO:0009607, GO:0020012, GO:0030682, GO:0042783, GO:0043207, GO:0044403, GO:0044413, GO:0044415, GO:0044419, GO:0050896 +13 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DUF3043 | PF11241.14 | 1.8e-56 | 48–232 | Protein of unknown function (DUF3043) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cobT (nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase), high confidence from genomic context alone (score 789 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2207 cobT |
nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase | 788 | 789 ctx | neighborhood:786 |
Rv2208 cobS |
adenosylcobinamide-GDP ribazoletransferase | 787 | 787 ctx | neighborhood:786 |
Rv2205c hyp |
hypothetical protein | 770 | 770 ctx | neighborhood:769 |
Rv2699c hyp |
hypothetical protein | 722 | 723 ctx | cooccurence:722 |
Rv2050 rbpA |
RNA polymerase-binding protein RbpA | 718 | 718 ctx | cooccurence:717 |
Rv2204c hyp |
hypothetical protein | 703 | 703 ctx | neighborhood:701 |
Rv2696c hyp |
hypothetical protein | 668 | 668 ctx | cooccurence:666 |
Rv0807 hyp |
hypothetical protein | 658 | 658 ctx | cooccurence:658 |
Rv2219 |
transmembrane protein | 647 | 647 ctx | cooccurence:596 |
Rv1830 |
HTH-type transcriptional regulator | 641 | 641 ctx | cooccurence:639 |
Rv2199c ctaF |
cytochrome c oxidase polypeptide 4 | 626 | 627 ctx | cooccurence:563 |
Rv2708c hyp |
hypothetical protein | 609 | 610 ctx | cooccurence:606 |
Rv3195 hyp |
hypothetical protein | 592 | 592 ctx | cooccurence:592 |
Rv1440 secG |
protein-export membrane protein SecG | 576 | 576 ctx | cooccurence:575 |
Rv2194 qcrC |
ubiquinol-cytochrome C reductase cytochrome subunit C | 550 | 550 ctx | cooccurence:548 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: DUF3043 domain-containing protein
- Pfam (hmmscan --cut_ga): DUF3043 PF11241.14 (E=2e-56)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216722.2)
- Domains: Pfam-A via hmmscan --cut_ga — DUF3043 (PF11241.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2AN86 - Curated reference: UniProt P9WLI5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
56 functional partner(s); context anchor
cobT - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002342|Rv2206| MKLLGHRKSHGHQRADASPDAGSKDGCRPDSGRTSGSDTSRGSQTTGPKGRPTPKRNQSRRHTKKGPVAPAPMTAAQARARRKSLAGPKLSREERRAEKAANRARMTERRERMMAGEEAYLLPRDRGPVRRYVRDVVDSRRNLLGLFMPSALTLLFVMFAVPQVQFYLSPAMLILLALMTIDAIILGRKVGRLVDTKFPSNTESRWRLGLYAAGRASQIRRLRAPRPQVERGGDVG