PPE38 Family assigned · medium auto-curated
H37Rv Rv2352c · MTBC0 - ·
391 aa · 2632923–2634098 (-) ·
RefSeq YP_177870.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | PPE family protein PPE38 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | PPE family protein PPE38. Pfam: PPE (PF00823.26), PPE-SVP (PF12484.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WHZ9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized PPE family protein PPE38 |
UniProt still lists this protein as Uncharacterized PPE family protein PPE38; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
N Cell motility
|
|---|---|
| eggNOG description | PPE family |
| Orthologous group | COG5651 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PPE | PF00823.26 | 1.1e-63 | 4–168 | PPE family |
PPE-SVP | PF12484.14 | 8.4e-10 | 299–387 | PPE-SVP subfamily C-terminal region |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2348c hyp |
hypothetical protein | 751 | 514 | coexpression:514 textmining:510 |
Rv1794 espG5 hyp |
hypothetical protein | 571 | 301 | textmining:412 |
Rv1040c PE8 |
PE family protein PE8 | 409 | 291 | |
Rv1793 esxN |
ESAT-6 like protein EsxN | 563 | 92 | textmining:539 |
Rv3874 esxB |
ESAT-6-like protein EsxB | 459 | 85 | textmining:434 |
Rv3875 esxA |
ESAT-6 protein EsxA | 462 | 70 | textmining:446 |
Rv2353c PPE39 |
PPE family protein PPE39 | 661 | 61 | textmining:654 |
Rv2248 hyp |
hypothetical protein | 555 | 57 | textmining:548 |
Rv2351c plcA |
membrane-associated phospholipase A | 656 | 49 | textmining:654 |
Rv0074 hyp |
hypothetical protein | 551 | 46 | textmining:549 |
Rv2350c plcB |
membrane-associated phospholipase B | 547 | 44 | textmining:546 |
Rv2349c plcC |
phospholipase C | 434 | 43 | textmining:433 |
Rv2741 PE_PGRS47 |
PE-PGRS family protein PE_PGRS47 | 625 | 41 | textmining:625 |
Rv1058 fadD14 |
fatty-acid--CoA ligase FadD14 | 541 | 41 | textmining:541 |
Rv1791 PE19 |
PE family protein PE19 | 511 | 41 | textmining:511 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): PPE family protein PPE38
- Pfam (hmmscan --cut_ga): PPE PF00823.26 (E=1e-63), PPE-SVP PF12484.14 (E=8e-10)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177870.1)
- Domains: Pfam-A via hmmscan --cut_ga — PPE (PF00823.26), PPE-SVP (PF12484.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG5651 - Curated reference: UniProt P9WHZ9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 15 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2352c|PPE38 MILDFSWLPPEINSARIYAGAGSGPLFMAAAAWEGLAADLRASASSFDAVIAGLAAGPWSGPASVAMAGAAAPYVGWLSAAAGQAELSAGQATAAATAFEAALAATVHPAAVTANRVLLGALVATNILGQNTPAIAATEFDYVEMWAQDVGAMVGYHAGAAAVAETLTPFSVPPLDLAGLASQAGAQLTGMATSVSAALSPIAEGAVEGVPAVVAAAQSVAAGLPVDAALQVGQAAAYPASMLIGPMMQLAQMGTTANTAGLAGAEAAGLAAADVPTFAGDIASGTGLGGAGGLGAGMSAELGKARLVGAMSVPPTWEGSVPARMASSAMAGLGAMPAEVPAAGGPMGMMPMPMGMGGAGAGMPAGMMGRGGANPHVVQARPSVVPRVGIG