bisC Resolved · high auto-curated
H37Rv Rv1442 · MTBC0 mtbc0_001543 ·
766 aa · 1629159–1631459 (+) ·
RefSeq NP_215958.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | biotin sulfoxide reductase BisC |
|---|---|
| MTBC0 PGAP re-annotation | molybdopterin guanine dinucleotide-containing S/N-oxide reductase |
| Revised (this work) | Molybdopterin guanine dinucleotide-containing S/N-oxide reductase. Pfam: Molybdopterin_N (PF18364.7), Molybdopterin (PF00384.28), Molydop_binding (PF01568.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06817
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable biotin sulfoxide reductase BisC |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | bisC |
| eggNOG description | Belongs to the prokaryotic molybdopterin-containing oxidoreductase family |
| Orthologous group | COG0243 |
| EC number |
EC 1.8.5.3, EC 1.97.1.9
|
| KEGG orthology |
K07306, K07309, K07310, K08351
|
| KEGG pathways |
map00450, map00780, map00920, map01100
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.033 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 12 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Molybdopterin_N | PF18364.7 | 3.0e-12 | 6–46 | Molybdopterin oxidoreductase N-terminal domain |
Molybdopterin | PF00384.28 | 8.6e-43 | 50–510 | Molybdopterin oxidoreductase |
Molydop_binding | PF01568.28 | 3.4e-22 | 623–739 | Molydopterin dinucleotide binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: PE_PGRS26 (PE-PGRS family protein PE_PGRS26), medium confidence from genomic context alone (score 593 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3279c birA |
bifunctional biotin operon repressor/biotin--[acetyl-CoA-carboxylase | 904 | 902 | database:900 |
Rv1589 bioB |
biotin synthetase | 900 | 900 | database:900 |
Rv2194 qcrC |
ubiquinol-cytochrome C reductase cytochrome subunit C | 816 | 797 | experimental:774 |
Rv0685 tuf |
elongation factor Tu | 807 | 791 | experimental:783 |
Rv1441c PE_PGRS26 |
PE-PGRS family protein PE_PGRS26 | 593 | 593 ctx | neighborhood:593 |
Rv3859c gltB |
glutamate synthase large subunit | 768 | 573 | experimental:420 textmining:481 |
Rv2195 qcrA |
ubiquinol-cytochrome C reductase rieske iron-sulfur subunit | 558 | 512 | experimental:484 |
Rv1162 narH |
nitrate reductase subunit beta | 637 | 458 | experimental:431 |
Rv3273 |
transmembrane carbonic anhydrase | 406 | 377 | |
Rv2899c fdhD |
formate dehydrogenase accessory protein FdhD | 407 | 343 | |
Rv3153 nuoI |
NADH-quinone oxidoreductase subunit I | 404 | 340 | |
Rv0984 moaB2 |
pterin-4-alpha-carbinolamine dehydratase | 561 | 223 | textmining:459 |
Rv0233 nrdB |
ribonucleoside-diphosphate reductase subunit beta NrdB | 719 | 214 | textmining:658 |
Rv2455c korA |
2-oxoglutarate oxidoreductase subunit KorA | 412 | 178 | |
Rv1857 modA |
molybdate ABC transporter substrate-binding lipoprotein ModA | 644 | 111 | textmining:616 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: biotin sulfoxide reductase BisC
- MTBC0 PGAP product: molybdopterin guanine dinucleotide-containing S/N-oxide reductase
- Pfam (hmmscan --cut_ga): Molybdopterin_N PF18364.7 (E=3e-12), Molybdopterin PF00384.28 (E=9e-43), Molydop_binding PF01568.28 (E=3e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215958.1)
- Domains: Pfam-A via hmmscan --cut_ga — Molybdopterin_N (PF18364.7), Molybdopterin (PF00384.28), Molydop_binding (PF01568.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0243 - Curated reference: UniProt O06817 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
PE_PGRS26 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001543|Rv1442|bisC MQVYTSATHWGVFTARVHGGDIAAVAALASDTNPAPQLQNLPGAVRHRSRIANPAVRRGWLQHGPGPSSARGAEEFVEVSWDELIELLASELRRTVDRYGNEAIYGSSYGWASAGRFHHAQSQVHRFLNMLGGYTASRHSYSAGASEVIFPHIVGAALFEALAETTTWDVIVDHTALLVAFGGLPVKNTAVMPGGTTAHPDRDYVGRYRARGGRLVSVSPLRDDIAAIAGPLDDRCRWLAPVPGTDVAIMLGLAYVLATESLADRAFLGRYCTGYERFERYLLGLDDGIPKTPEWAAALSGLAAGDLRDLARRMAEHRTLITTSLSLQRIEHGEQTVWMAATLAAMLGQIGLPGGGFGHGYSSNGVGNPPLACGLPALPQGNNPVSTFIPVAAISELLQRPGQRLAYNGRLLELPDIKCVYWAGGNPFHHHQNLPRLRRALSRVDTIVVHEQYWTAMAKHADIVVPTTTSFERDDFAASKTNPTLIAMPAMVPPYANARDDYHTFSALAHRLGFGKQFTEGRSAREWLEHMYDKWSAELDFPVPSFAEFWRTGRLELPTRTGLTWLADFRADPAAHPLGTPSGRIEIFSDTVDAFALPDCAGHPTWYEPSEWLGGPRAARYPLHLIANQPRTRLHSQLDHGGASMASKIRGREPIRIHPDDAAARELTDGDIVRVFNDRGACLAGVVIDDGLRPKVVQLSTGAWFDPADPRDPDSMCVHGNPNALSNDSGTSSLAHGSTGQHVLVQIERFTGELPPVRAHEPPRLA