zwf2 Resolved · high auto-curated
H37Rv Rv1447c · MTBC0 mtbc0_001549 ·
514 aa · 1634786–1636330 (-) ·
RefSeq NP_215963.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | glucose-6-phosphate 1-dehydrogenase |
|---|---|
| MTBC0 PGAP re-annotation | glucose-6-phosphate dehydrogenase |
| Revised (this work) | Glucose-6-phosphate dehydrogenase. Pfam: G6PD_N (PF00479.29), G6PD_C (PF02781.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WN73
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Glucose-6-phosphate 1-dehydrogenase 2 |
| EC (curated) |
EC 1.1.1.49
|
| Curated function | Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | zwf |
| eggNOG description | Catalyzes the oxidation of glucose 6-phosphate to 6- phosphogluconolactone |
| Orthologous group | COG0364 |
| EC number |
EC 1.1.1.363, EC 1.1.1.49
|
| KEGG orthology |
K00036
|
| KEGG pathways |
map00030, map00480, map01100, map01110, map01120, map01130, map01200, map05230
|
| KEGG modules |
M00004, M00006, M00008
|
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.328 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 7 synonymous, 6 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 1.45% of strains (2106) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
G6PD_N | PF00479.29 | 2.3e-65 | 32–215 | Glucose-6-phosphate dehydrogenase, NAD binding domain |
G6PD_C | PF02781.22 | 6.1e-114 | 217–512 | Glucose-6-phosphate dehydrogenase, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: devB (6-phosphogluconolactonase), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1445c devB |
6-phosphogluconolactonase | 999 | 1000 ctx | neighborhood:811 fusion:899 cooccurence:762 coexpression:691 database:900 textmining:711 |
Rv1448c tal |
transaldolase | 983 | 966 ctx | neighborhood:881 coexpression:521 textmining:516 |
Rv0946c pgi |
glucose-6-phosphate isomerase | 977 | 952 | coexpression:480 database:900 textmining:550 |
Rv1449c tkt |
transketolase | 982 | 949 ctx | neighborhood:865 coexpression:439 textmining:674 |
Rv1844c gnd1 |
6-phosphogluconate dehydrogenase | 962 | 927 ctx | cooccurence:723 coexpression:659 textmining:505 |
Rv1446c opcA |
OXPP cycle protein OpcA | 955 | 927 ctx | neighborhood:811 coexpression:417 textmining:419 |
Rv1121 zwf1 |
glucose-6-phosphate 1-dehydrogenase | 926 | 916 | database:900 |
Rv1122 gnd2 |
6-phosphogluconate dehydrogenase (decarboxylating) | 935 | 910 ctx | fusion:643 cooccurence:718 |
Rv1436 gap |
glyceraldehyde 3-phosphate dehydrogenase | 953 | 898 | coexpression:428 database:800 textmining:560 |
Rv3068c pgmA |
phosphoglucomutase PgmA | 933 | 890 | database:800 textmining:419 |
Rv1617 pykA |
pyruvate kinase | 915 | 847 | database:800 textmining:470 |
Rv0489 gpm1 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 850 | 831 | database:800 |
Rv0363c fba |
fructose-bisphosphate aldolase | 865 | 815 | database:800 |
Rv1438 tpi |
triosephosphate isomerase | 891 | 814 | database:800 textmining:440 |
Rv2702 ppgK |
polyphosphate glucokinase | 848 | 811 | database:800 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: glucose-6-phosphate 1-dehydrogenase
- MTBC0 PGAP product: glucose-6-phosphate dehydrogenase
- Pfam (hmmscan --cut_ga): G6PD_N PF00479.29 (E=2e-65), G6PD_C PF02781.22 (E=6e-114)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215963.1)
- Domains: Pfam-A via hmmscan --cut_ga — G6PD_N (PF00479.29), G6PD_C (PF02781.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0364 - Curated reference: UniProt P9WN73 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
49 functional partner(s); context anchor
devB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001549|Rv1447c|zwf2 MKPAHAAASWRNPLRDKRDKRLPRIAGPCGMVIFGVTGDLARKKVMPAVYDLANRGLLPPTFSLVGFARRDWSTQDFGQVVYNAVQEHCRTPFRQQNWDRLAEGFRFVPGTFDDDDAFAQLAETLEKLDAERGTGGNHAFYLAIPPKSFPVVCEQLHKSGLARPQGDRWSRVVIEKPFGHDLASARELNKAVNAVFPEEAVFRIDHYLGKETVQNILALRFANQLFDPIWNAHYVDHVQITMAEDIGLGGRAGYYDGIGAARDVIQNHLMQLLALTAMEEPVSFHPAALQAEKIKVLSATRLAEPLDQTTSRGQYAAGWQGGEKVVGLLDEEGFAEDSTTETFAAITLEVDTRRWAGVPFYLRTGKRLGRRVTEIALVFRRAPHLPFDATMTDELGTNAMVIRVQPDEGVTLRFGSKVPGTAMEVRDVNMDFSYGSAFAEDSPEAYERLILDVLLGEPSLFPVNAEVELAWEILDPALEHWAAHGTPDAYEAGTWGPESSLEMLRRTGREWRRP