lprH Family assigned · low
H37Rv Rv1418 · MTBC0 mtbc0_001518 ·
228 aa · 1601983–1602669 (+) ·
RefSeq NP_215934.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | lipoprotein LprH |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Lipoprotein LprH; a TLR2-stimulating lipoprotein of the M. tuberculosis complex (its over-expression enhances TLR2-dependent HIV infectivity of CD4+ T cells). Cell-envelope lipoprotein; precise molecular function unfixed. |
Curated reference (UniProt)
| UniProt |
P9WK43
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative lipoprotein LprH |
UniProt still lists this protein as Putative lipoprotein LprH; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Preferred name | lprH |
|---|---|
| Orthologous group | 2B4WT |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.476 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ribH (6,7-dimethyl-8-ribityllumazine synthase), high confidence from genomic context alone (score 851 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1416 ribH |
6,7-dimethyl-8-ribityllumazine synthase | 850 | 851 ctx | neighborhood:846 |
Rv1417 |
membrane protein | 849 | 850 ctx | neighborhood:846 |
Rv1415 ribA2 |
bifunctional riboflavin biosynthesis GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase | 826 | 827 ctx | neighborhood:824 |
Rv1419 hyp |
hypothetical protein | 486 | 487 ctx | neighborhood:485 |
Rv1413 hyp |
hypothetical protein | 478 | 478 ctx | neighborhood:475 |
Rv1414 hyp |
hypothetical protein | 478 | 478 ctx | neighborhood:475 |
Rv1421 rapZ hyp |
hypothetical protein | 442 | 442 ctx | neighborhood:435 |
Rv1423 whiA |
transcriptional regulator WhiA | 439 | 439 ctx | neighborhood:435 |
Rv1422 cuvA hyp |
hypothetical protein | 439 | 439 ctx | neighborhood:435 |
Rv1420 uvrC |
excinuclease ABC subunit UvrC | 437 | 437 ctx | neighborhood:435 |
Rv3576 lppH |
lipoprotein LppH | 861 | 50 | textmining:860 |
Rv3016 lpqA |
lipoprotein LpqA | 870 | 45 | textmining:870 |
Rv3705c hyp |
hypothetical protein | 870 | 44 | textmining:870 |
Rv0835 lpqQ |
lipoprotein LpqQ | 870 | 44 | textmining:870 |
Rv1266c pknH |
serine/threonine-protein kinase PknH | 514 | 41 | textmining:514 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- TLR2-modulating cell-envelope lipoprotein (Skerry 2016, PMID 26807859)
- Curated from the literature crible (project 'Still unknown gene function', 2026-06-09)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215934.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2B4WT - Curated reference: UniProt P9WK43 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
15 functional partner(s); context anchor
ribH - Primary literature: Skerry C, Klinkenberg LG, Page KR, Karakousis PC (2016). TLR2-Modulating Lipoproteins of the Mycobacterium tuberculosis Complex Enhance the HIV Infectivity of CD4+ T Cells PLoS One 11(1):e0147192. doi:10.1371/journal.pone.0147192 PMID:26807859
Ancestral MTBC0 protein sequence
>mtbc0_001518|Rv1418|lprH MACLGRPGCRGWAGASLVLVVVLALAACTESVAGRAMRATDRSSGLPTSAKPARARDLLLQDGDRAPFGQVTQSRVGDSYFTSAVPPECSAALLFKGSPLRPDGSSDHAEAAYNVTGPLPYAESVDVYTNVLNVHDVVWNGFRDVSHCRGDAVGVSRAGRSTPMRLRYFATLSDGVLVWTMSNPRWTCDYGLAVVPHAVLVLSACGFKPGFPMAEWASKRRAQLDSQV