Rv0738 Family assigned · medium auto-curated
H37Rv Rv0738 · MTBC0 mtbc0_000782 ·
182 aa · 834255–834803 (+) ·
RefSeq NP_215252.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | TIGR03086 family metal-binding protein |
| Revised (this work) | TIGR03086 family metal-binding protein. Pfam: MDMPI_N (PF11716.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WKS3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv0738 |
UniProt still lists this protein as Uncharacterized protein Rv0738; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesis
|
|---|---|
| eggNOG description | Mycothiol maleylpyruvate isomerase N-terminal domain |
| Orthologous group | COG1576 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.177 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MDMPI_N | PF11716.14 | 4.4e-12 | 9–121 | Mycothiol maleylpyruvate isomerase N-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0802c (succinyl-CoA transferase), medium confidence from genomic context alone (score 546 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2420c rsfS hyp |
hypothetical protein | 792 | 765 | coexpression:746 |
Rv3780 bpa hyp |
hypothetical protein | 567 | 567 ctx | cooccurence:566 |
Rv3773c hyp |
hypothetical protein | 556 | 556 ctx | cooccurence:552 |
Rv0802c |
succinyl-CoA transferase | 546 | 546 ctx | cooccurence:542 |
Rv3859c gltB |
glutamate synthase large subunit | 544 | 544 ctx | neighborhood:544 |
Rv0736 rslA |
anti-sigma-L factor RslA | 515 | 515 | |
Rv2735c hyp |
hypothetical protein | 472 | 472 ctx | cooccurence:472 |
Rv0737 |
transcriptional regulator | 492 | 467 ctx | neighborhood:467 |
Rv2421c nadD |
nicotinate-nucleotide adenylyltransferase | 539 | 461 | coexpression:417 |
Rv0739 hyp |
hypothetical protein | 448 | 448 ctx | neighborhood:443 |
Rv1354c hyp |
hypothetical protein | 406 | 407 | |
Rv0194 |
multidrug ABC transporter ATPase/permease | 405 | 406 | |
Rv0080 hyp |
hypothetical protein | 405 | 405 | |
Rv0613c hyp |
hypothetical protein | 404 | 404 | |
Rv1416 ribH |
6,7-dimethyl-8-ribityllumazine synthase | 840 | 226 | textmining:803 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: TIGR03086 family metal-binding protein
- Pfam (hmmscan --cut_ga): MDMPI_N PF11716.14 (E=4e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215252.1)
- Domains: Pfam-A via hmmscan --cut_ga — MDMPI_N (PF11716.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1576 - Curated reference: UniProt P9WKS3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
19 functional partner(s); context anchor
Rv0802c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000782|Rv0738| MDPLMAHQRAQDAFAALLANVRADQLGGPTPCSEWTINDLIEHVVGGNEQVGRWAASPIEPPARPDGLVAAHQAAAAVAHEIFAAPGGMSATFKLPLGEVPGQVFIGLRTTDVLTHAWDLAAATGQSTDLDPELAVERLAAARALVGPQFRGPGKPFADEKPCPRERPPADQLAAFLGRTVR