ftsX Resolved · high auto-curated
H37Rv Rv3101c · MTBC0 mtbc0_003297 ·
297 aa · 3491150–3492043 (-) ·
RefSeq NP_217617.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell division protein FtsX |
|---|---|
| MTBC0 PGAP re-annotation | permease-like cell division protein FtsX |
| Revised (this work) | Permease-like cell division protein FtsX. Pfam: FtsX_ECD (PF18075.9), FtsX (PF02687.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WG19
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell division protein FtsX |
| Curated function | Part of the ABC transporter FtsEX involved in cellular division. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | ftsX |
| eggNOG description | Part of the ABC transporter FtsEX involved in cellular division |
| Orthologous group | COG2177 |
| KEGG orthology |
K09811
|
| KEGG pathways |
map02010
|
| KEGG modules |
M00256
|
| Gene Ontology (19) |
GO:0005575, GO:0005576, GO:0005618, GO:0005623, GO:0005886, GO:0005887, GO:0008150, GO:0009987, GO:0016020, GO:0016021, GO:0030312, GO:0031224 +7 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.332 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FtsX_ECD | PF18075.9 | 2.6e-21 | 56–166 | FtsX extracellular domain |
FtsX | PF02687.28 | 1.2e-14 | 175–293 | FtsX-like permease C-terminal |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ftsE (cell division ATP-binding protein FtsE), high confidence from genomic context alone (score 997 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3102c ftsE |
cell division ATP-binding protein FtsE | 999 | 997 ctx | neighborhood:882 cooccurence:764 coexpression:892 textmining:974 |
Rv3100c smpB |
SsrA-binding protein | 960 | 896 ctx | neighborhood:882 textmining:630 |
Rv3099c hyp |
hypothetical protein | 832 | 832 ctx | neighborhood:830 |
Rv3105c prfB |
peptide chain release factor PrfB | 778 | 709 ctx | neighborhood:706 |
Rv3104c |
transmembrane protein | 706 | 706 ctx | neighborhood:702 |
Rv3103c hyp |
hypothetical protein | 690 | 690 ctx | neighborhood:682 |
Rv1631 coaE |
dephospho-CoA kinase CoaE | 533 | 533 | coexpression:439 |
Rv3106 fprA |
NADPH-ferredoxin reductase FprA | 526 | 526 ctx | neighborhood:525 |
Rv0950c hyp |
hypothetical protein | 490 | 453 | |
Rv1477 ripA |
peptidoglycan endopeptidase RipA | 610 | 449 | |
Rv2553c mltG |
membrane protein | 445 | 421 | |
Rv1239c corA |
magnesium and cobalt transport transmembrane protein CorA | 421 | 421 | coexpression:402 |
Rv3915 cwlM |
peptidoglycan hydrolase | 578 | 407 | |
Rv1709 scpA |
segregation and condensation protein ScpA | 402 | 403 | |
Rv2145c wag31 |
cell wall synthesis protein Wag31 | 494 | 390 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cell division protein FtsX
- MTBC0 PGAP product: permease-like cell division protein FtsX
- Pfam (hmmscan --cut_ga): FtsX_ECD PF18075.9 (E=3e-21), FtsX PF02687.28 (E=1e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217617.1)
- Domains: Pfam-A via hmmscan --cut_ga — FtsX_ECD (PF18075.9), FtsX (PF02687.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2177 - Curated reference: UniProt P9WG19 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
31 functional partner(s); context anchor
ftsE - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003297|Rv3101c|ftsX MRFGFLLNEVLTGFRRNVTMTIAMILTTAISVGLFGGGMLVVRLADSSRAIYLDRVESQVFLTEDVSANDSSCDTTACKALREKIETRSDVKAVRFLNRQQAYDDAIRKFPQFKDVAGKDSFPASFIVKLENPEQHKDFDTAMKGQPGVLDVLNQKELIDRLFAVLDGLSNAAFAVALVQAIGAILLIANMVQVAAYTRRTEIGIMRLVGASRWYTQLPFLVEAMLAATMGVGIAVAGLMVVRALFLENALNQFYQANLIAKVDYADILFITPWLLLLGVAMSGLTAYLTLRLYVRR