Rv0950c Family assigned · medium auto-curated
H37Rv Rv0950c · MTBC0 mtbc0_001014 ·
332 aa · 1067871–1068869 (-) ·
RefSeq NP_215465.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | M23 family metallopeptidase |
| Revised (this work) | M23 family metallopeptidase. Pfam: Peptidase_M23 (PF01551.30). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71560
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | M23ase beta-sheet core domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | mepA |
| eggNOG description | peptidase |
| Orthologous group | COG0739 |
| KEGG orthology |
K21471
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.234 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Peptidase_M23 | PF01551.30 | 8.2e-31 | 222–316 | Peptidase family M23 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: sucC (succinyl-CoA ligase subunit beta), medium confidence from genomic context alone (score 556 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1886c fbpB |
diacylglycerol acyltransferase/mycolyltransferase Ag85B | 868 | 859 | coexpression:859 |
Rv1885c |
chorismate mutase | 804 | 804 | coexpression:804 |
Rv2190c ripC |
endopeptidase | 882 | 795 | coexpression:761 textmining:453 |
Rv0951 sucC |
succinyl-CoA ligase subunit beta | 686 | 556 ctx | neighborhood:467 |
Rv3682 ponA2 |
bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase | 588 | 543 ctx | cooccurence:527 |
Rv2869c rip |
zinc metalloprotease | 515 | 467 ctx | cooccurence:425 |
Rv3101c ftsX |
cell division protein FtsX | 490 | 453 | |
Rv0952 sucD |
succinyl-CoA ligase subunit alpha | 451 | 451 ctx | neighborhood:416 |
Rv2903c lepB |
signal peptidase | 471 | 439 | |
Rv2586c secF |
protein translocase subunit SecF | 455 | 418 | |
Rv1009 rpfB |
resuscitation-promoting factor RpfB | 547 | 415 | |
Rv0050 ponA1 |
bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase | 424 | 401 | |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 417 | 386 | |
Rv2553c mltG |
membrane protein | 412 | 375 | |
Rv2154c ftsW |
lipid II flippase FtsW | 409 | 373 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: M23 family metallopeptidase
- Pfam (hmmscan --cut_ga): Peptidase_M23 PF01551.30 (E=8e-31)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215465.1)
- Domains: Pfam-A via hmmscan --cut_ga — Peptidase_M23 (PF01551.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0739 - Curated reference: UniProt P71560 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
28 functional partner(s); context anchor
sucC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001014|Rv0950c| MAAIRTPRDRWPHHHRNEVTEIIPLDGFLDGLALYDELDFAELDDLDLGDDCVFDYEAQLLAAPELDDLDDADDLAPEWLVAPTVVLTPEVTPVSRRVGQHRKQPIGAARGRLLISAMAAGAAAAAAHTAIQQSETPRTETVLTAHASALNEGSGSNPPRGVQVIAAQPAASAAVHNAEFARGVAFAEERAEREARLQRPLYVMPTKGIFTSSFGYRWGVLHAGIDLANAIGTPIYAVSDGVVIDAGPTAGYGMWVKLLHADGTVTLYGHVNTTLVSVGERVMAGDQIATMGSRGFSTGPHLHFEVLLGGTERVDPVPWLAKRGLSVGNYTG