Rv3371 Resolved · high auto-curated
H37Rv Rv3371 · MTBC0 mtbc0_003586 ·
446 aa · 3811649–3812989 (+) ·
RefSeq NP_217888.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | diacyglycerol O-acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | diacylglycerol O-acyltransferase |
| Revised (this work) | Diacylglycerol O-acyltransferase. Pfam: WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WKA9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative diacyglycerol O-acyltransferase Rv3371 |
| EC (curated) |
EC 2.3.1.20
|
| Curated function | Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis..; FUNCTION: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters. |
UniProt still lists this protein as Putative diacyglycerol O-acyltransferase Rv3371; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
Q Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| eggNOG description | Wax ester synthase-like Acyl-CoA acyltransferase domain |
| Orthologous group | COG1020 |
| Gene Ontology (60) |
GO:0000302, GO:0001666, GO:0003674, GO:0003824, GO:0004144, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0006629, GO:0006638, GO:0006639 +48 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 4.938 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 14 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WS_DGAT_cat | PF03007.22 | 1.4e-41 | 4–237 | Wax ester synthase/diacylglycerol acyltransferase catalytic domain |
WS_DGAT_C | PF06974.19 | 1.6e-14 | 272–399 | WS/DGAT C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3233c (Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13), high confidence from genomic context alone (score 772 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3097c lipY |
triacylglycerol lipase Lip | 912 | 913 | database:900 |
Rv2252 dagK |
diacylglycerol kinase | 900 | 900 | database:900 |
Rv3233c |
Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13 | 839 | 772 ctx | cooccurence:772 |
Rv3372 otsB2 |
trehalose 6-phosphate phosphatase | 737 | 726 ctx | neighborhood:682 |
Rv2627c hyp |
hypothetical protein | 678 | 667 ctx | cooccurence:505 |
Rv1192 hyp |
hypothetical protein | 654 | 654 ctx | cooccurence:652 |
Rv1543 |
oxidoreductase | 670 | 547 ctx | cooccurence:536 |
Rv3370c dnaE2 |
error-prone DNA polymerase | 521 | 521 ctx | neighborhood:500 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 572 | 506 | experimental:441 |
Rv0547c |
oxidoreductase | 505 | 505 ctx | cooccurence:493 |
Rv2933 ppsC |
phthiocerol synthesis polyketide synthase type I PpsC | 531 | 504 | experimental:441 |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 608 | 501 | experimental:441 |
Rv2048c pks12 |
polyketide synthase | 527 | 500 | experimental:441 |
Rv1527c pks5 |
polyketide synthase | 526 | 498 | experimental:441 |
Rv3391 acrA1 |
acyl-CoA-reductase AcrA | 752 | 493 ctx | cooccurence:459 textmining:531 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: diacyglycerol O-acyltransferase
- MTBC0 PGAP product: diacylglycerol O-acyltransferase
- Pfam (hmmscan --cut_ga): WS_DGAT_cat PF03007.22 (E=1e-41), WS_DGAT_C PF06974.19 (E=2e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217888.1)
- Domains: Pfam-A via hmmscan --cut_ga — WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1020 - Curated reference: UniProt P9WKA9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
42 functional partner(s); context anchor
Rv3233c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003586|Rv3371| MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVALPRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQIPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAAITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIHALARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPTALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIELGVARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH