Rv3480c Family assigned · medium auto-curated
H37Rv Rv3480c · MTBC0 mtbc0_003695 ·
497 aa · 3923491–3924984 (-) ·
RefSeq NP_217997.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | diacyglycerol O-acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | wax ester/triacylglycerol synthase family O-acyltransferase |
| Revised (this work) | Wax ester/triacylglycerol synthase family O-acyltransferase. Pfam: WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WKA7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative diacyglycerol O-acyltransferase Rv3480c |
| EC (curated) |
EC 2.3.1.20
|
| Curated function | Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. |
UniProt still lists this protein as Putative diacyglycerol O-acyltransferase Rv3480c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Belongs to the long-chain O-acyltransferase family |
| Orthologous group | COG1020 |
| Gene Ontology (60) |
GO:0000302, GO:0001666, GO:0003674, GO:0003824, GO:0004144, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0006629, GO:0006638, GO:0006639 +48 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 1.204 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 7 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 1.41% of strains (2046) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WS_DGAT_cat | PF03007.22 | 1.6e-89 | 8–298 | Wax ester synthase/diacylglycerol acyltransferase catalytic domain |
WS_DGAT_C | PF06974.19 | 4.8e-36 | 338–486 | WS/DGAT C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: acrA1 (acyl-CoA-reductase AcrA), high confidence from genomic context alone (score 824 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3097c lipY |
triacylglycerol lipase Lip | 926 | 925 | database:900 |
Rv2252 dagK |
diacylglycerol kinase | 902 | 903 | database:900 |
Rv3391 acrA1 |
acyl-CoA-reductase AcrA | 943 | 824 ctx | cooccurence:693 coexpression:419 textmining:692 |
Rv3800c pks13 |
polyketide synthase | 808 | 745 | coexpression:709 |
Rv2383c mbtB |
phenyloxazoline synthase | 795 | 712 | coexpression:650 |
Rv1543 |
oxidoreductase | 857 | 711 ctx | cooccurence:695 textmining:528 |
Rv2627c hyp |
hypothetical protein | 673 | 673 ctx | cooccurence:662 |
Rv0547c |
oxidoreductase | 658 | 659 ctx | cooccurence:640 |
Rv1192 hyp |
hypothetical protein | 605 | 605 ctx | cooccurence:603 |
Rv3481c |
integral membrane protein | 591 | 591 ctx | neighborhood:591 |
Rv3775 lipE |
lipase LipE | 521 | 521 ctx | cooccurence:506 |
Rv0101 nrp |
peptide synthetase Nrp | 543 | 518 | coexpression:481 |
Rv0646c lipG |
lipase/esterase LipG | 485 | 485 ctx | cooccurence:485 |
Rv2590 fadD9 |
fatty-acid--CoA ligase FadD9 | 501 | 483 | coexpression:409 |
Rv0945 |
oxidoreductase | 456 | 457 ctx | cooccurence:427 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: diacyglycerol O-acyltransferase
- MTBC0 PGAP product: wax ester/triacylglycerol synthase family O-acyltransferase
- Pfam (hmmscan --cut_ga): WS_DGAT_cat PF03007.22 (E=2e-89), WS_DGAT_C PF06974.19 (E=5e-36)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217997.1)
- Domains: Pfam-A via hmmscan --cut_ga — WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1020 - Curated reference: UniProt P9WKA7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
39 functional partner(s); context anchor
acrA1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003695|Rv3480c| MSQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVDDNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLSTDPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVNAQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALAIIGGGLRRFLDELGELPNKSLIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQLAAADGAAGLGS