Rv2766c Resolved · high auto-curated
H37Rv Rv2766c · MTBC0 - ·
260 aa · 3075588–3076370 (-) ·
RefSeq YP_177905.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | short-chain type dehydrogenase/reductase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Short-chain type dehydrogenase/reductase. Pfam: SDR (PF23441.1), adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
I6YEB6
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable short-chain type dehydrogenase/reductase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| eggNOG description | Enoyl-(Acyl carrier protein) reductase |
| Orthologous group | COG1028 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.531 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 2 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.14% of strains (202) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
SDR | PF23441.1 | 3.7e-11 | 6–246 | SDR-like rossmann domain |
adh_short | PF00106.32 | 3.7e-50 | 10–198 | short chain dehydrogenase |
KR | PF08659.17 | 1.0e-06 | 10–162 | KR domain |
adh_short_C2 | PF13561.13 | 4.3e-59 | 15–244 | Enoyl-(Acyl carrier protein) reductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2767c (membrane protein), high confidence from genomic context alone (score 784 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2524c fas |
fatty acid synthase | 827 | 802 | coexpression:509 experimental:475 |
Rv2767c |
membrane protein | 784 | 784 ctx | neighborhood:781 |
Rv1933c fadE18 |
acyl-CoA dehydrogenase FadE18 | 707 | 697 ctx | neighborhood:544 |
Rv3761c fadE36 |
acyl-CoA dehydrogenase FadE36 | 703 | 692 ctx | neighborhood:544 |
Rv1882c |
short-chain type dehydrogenase/reductase | 681 | 682 ctx | cooccurence:675 |
Rv0945 |
oxidoreductase | 594 | 579 ctx | cooccurence:520 |
Rv0148 |
short-chain type dehydrogenase/reductase | 607 | 558 ctx | cooccurence:557 |
Rv1937 |
oxygenase | 593 | 546 | |
Rv2243 fabD |
malonyl CoA-acyl carrier protein transacylase | 552 | 536 | |
Rv2299c htpG |
chaperone protein HtpG | 545 | 525 | database:450 |
Rv2564 glnQ |
glutamine ABC transporter ATP-binding protein | 536 | 515 | database:431 |
Rv0073 |
glutamine ABC transporter ATP-binding protein | 532 | 510 | database:431 |
Rv3728 |
membrane protein | 534 | 509 | database:431 |
Rv3239c |
transmembrane transport protein | 532 | 507 | database:431 |
Rv2565 |
NTE family protein | 521 | 502 | database:431 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): short-chain type dehydrogenase/reductase
- Pfam (hmmscan --cut_ga): SDR PF23441.1 (E=4e-11), adh_short PF00106.32 (E=4e-50), KR PF08659.17 (E=1e-06), adh_short_C2 PF13561.13 (E=4e-59)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177905.1)
- Domains: Pfam-A via hmmscan --cut_ga — SDR (PF23441.1), adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1028 - Curated reference: UniProt I6YEB6 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
78 functional partner(s); context anchor
Rv2767c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2766c| MTSLDLTGRTAIITGASRGIGLAIAQQLAAAGAHVVLTARRQEAADEAAAQVGDRALGVGAHAVDEDAARRCVDLTLERFGSVDILINNAGTNPAYGPLLEQDHARFAKIFDVNLWAPLMWTSLVVTAWMGEHGGAVVNTASIGGMHQSPAMGMYNATKAALIHVTKQLALELSPRIRVNAICPGVVRTRLAEALWKDHEDPLAATIALGRIGEPADIASAVAFLVSDAASWITGETMIIDGGLLLGNALGFRAAPSTEH