eccC5 Resolved · high auto-curated

H37Rv Rv1783 · MTBC0 - · 1391 aa · 2019257–2023432 (+) · RefSeq YP_007410461.1

Annotation: from legacy to revised

Legacy (H37Rv / Mycobrowser)ESX-5 type VII secretion system protein EccC5
MTBC0 PGAP re-annotation
Revised (this work)ESX-5 type VII secretion system protein EccC5. Pfam: FtsK_SpoIIIE (PF01580.25).

Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.

Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).

Curated reference (UniProt)

UniProt P9WNA5 SwissProt · reviewed · Evidence at protein level
UniProt nameESX-5 secretion system protein EccC5
Curated functionPart of the ESX-5 specialized secretion system, which is responsible for the secretion of EsxN and a number of PE_PGRS and PPE proteins, including PPE41.

Functional vocabulary (eggNOG-mapper, orthology transfer)

COG category D Cell cycle control, cell division, chromosome partitioning
Preferred nameeccCa
eggNOG descriptionSecretion protein
Orthologous groupCOG1674
KEGG orthology K03466
Gene Ontology (8) GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0016020, GO:0030312, GO:0044464, GO:0071944

Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.

Conservation & selection (intra-MTBC, 145 209 strains)

pN/pS 0.204 · purifying
Polymorphic sites (≥ 0.1% of strains) 16 synonymous, 10 missense, 0 nonsense, 0 frameshift

pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.

Domains (Pfam, hmmscan --cut_ga)

PfamAccessioni-EvalueResiduesDescription
FtsK_SpoIIIEPF01580.25 1.9e-61824–1096 FtsK/SpoIIIE family

Functional interaction network (STRING v12, guilt-by-association)

Closest characterised functional partner: eccD5 (ESX-5 type VII secretion system protein EccD), high confidence from genomic context alone (score 1000 excluding text-mining).

PartnerProductScoreNo text-miningChannels (≥400)
Rv1795 eccD5 ESX-5 type VII secretion system protein EccD 999 1000 ctx cooccurence:761 experimental:999 textmining:988
Rv1782 eccB5 ESX-5 type VII secretion system protein EccB5 999 1000 ctx neighborhood:881 cooccurence:771 coexpression:804 experimental:999 textmining:977
Rv1796 mycP5 membrane-anchored mycosin MycP 984 940 ctx cooccurence:698 experimental:800 textmining:752
Rv0283 eccB3 ESX-3 secretion system protein EccB3 933 923 ctx cooccurence:561 experimental:824
Rv0290 eccD3 ESX-3 secretion system protein EccD 891 875 experimental:821
Rv3448 eccD4 ESX-4 secretion system protein EccD4 882 870 ctx cooccurence:734 experimental:513
Rv3895c eccB2 ESX-2 secretion system protein EccB 842 833 ctx cooccurence:767
Rv3887c eccD2 ESX-2 secretion system protein EccD 877 781 ctx cooccurence:637 textmining:462
Rv3445c esxU ESAT-6 like protein EsxU 732 698 experimental:677
Rv3874 esxB ESAT-6-like protein EsxB 860 691 experimental:677 textmining:568
Rv3450c eccB4 ESX-4 secretion system protein EccB4 677 641 ctx cooccurence:501
Rv2151c ftsQ cell division protein FtsQ 627 604 experimental:401
Rv0292 eccE3 ESX-3 secretion system protein EccE 629 575 experimental:571
Rv3886c mycP2 membrane-anchored mycosin 593 541 ctx cooccurence:517
Rv1781c malQ 4-alpha-glucanotransferase 593 508 ctx neighborhood:505

STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.

Evidence

  • Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): ESX-5 type VII secretion system protein EccC5
  • Pfam (hmmscan --cut_ga): FtsK_SpoIIIE PF01580.25 (E=2e-61)
  • (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)

Sources

  • Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
  • Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_007410461.1)
  • Domains: Pfam-A via hmmscan --cut_ga — FtsK_SpoIIIE (PF01580.25)
  • Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
  • Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021, doi:10.1093/molbev/msab293), eggNOG 5.0 DB (Huerta-Cepas et al. 2019) — OG COG1674
  • Curated reference: UniProt P9WNA5 (SwissProt, reviewed; Evidence at protein level)
  • Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
  • Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 47 functional partner(s); context anchor eccD5
  • Primary literature: none located yet; annotation rests on the domain/homology sources above.

Ancestral MTBC0 protein sequence

>H37Rv|Rv1783|eccC5
MKRGFARPTPEKPPVIKPENIVLSTPLSIPPPEGKPWWLIVVGVVVVGLLGGMVAMVFASGSHVFGGIGSIFPLFMMVGIMMMMFRGMGGGQQQMSRPKLDAMRAQFMLMLDMLRETAQESADSMDANYRWFHPAPNTLAAAVGSPRMWERKPDGKDLNFGVVRVGVGMTRPEVTWGEPQNMPTDIELEPVTGKALQEFGRYQSVVYNLPKMVSLLVEPWYALVGEREQVLGLMRAIICQLAFSHGPDHVQMIVVSSDLDQWDWVKWLPHFGDSRRHDAAGNARMVYTSVREFAAEQAELFAGRGSFTPRHASSSAQTPTPHTVIIADVDDPQWEYVISAEGVDGVTFFDLTGSSMWTDIPERKLQFDKTGVIEALPRDRDTWMVIDDKAWFFALTDQVSIAEAEEFAQKLAQWRLAEAYEEIGQRVAHIGARDILSYYGIDDPGNIDFDSLWASRTDTMGRSRLRAPFGNRSDNGELLFLDMKSLDEGGDGPHGVMSGTTGSGKSTLVRTVIESLMLSHPPEELQFVLADLKGGSAVKPFAGVPHVSRIITDLEEDQALMERFLDALWGEIARRKAICDSAGVDDAKEYNSVRARMRARGQDMAPLPMLVVVIDEFYEWFRIMPTAVDVLDSIGRQGRAYWIHLMMASQTIESRAEKLMENMGYRLVLKARTAGAAQAAGVPNAVNLPAQAGLGYFRKSLEDIIRFQAEFLWRDYFQPGVSIDGEEAPALVHSIDYIRPQLFTNSFTPLEVSVGGPDIEPVVAQPNGEVLESDDIEGGEDEDEEGVRTPKVGTVIIDQLRKIKFEPYRLWQPPLTQPVAIDDLVNRFLGRPWHKEYGSACNLVFPIGIIDRPYKHDQPPWTVDTSGPGANVLILGAGGSGKTTALQTLICSAALTHTPQQVQFYCLAYSSTALTTVSRIPHVGEVAGPTDPYGVRRTVAELLALVRERKRSFLECGIASMEMFRRRKFGGEAGPVPDDGFGDVYLVIDNYRALAEENEVLIEQVNVIINQGPSFGVHVVVTADRESELRPPVRSGFGSRIELRLAAVEDAKLVRSRFAKDVPVKPGRGMVAVNYVRLDSDPQAGLHTLVARPALGSTPDNVFECDSVVAAVSRLTSAQAPPVRRLPARFGVEQVRELASRDTRQGVGAGGIAWAISELDLAPVYLNFAENSHLMVTGRRECGRTTTLATIMSEIGRLYAPGASSAPPPAPGRPSAQVWLVDPRRQLLTALGSDYVERFAYNLDGVVAMMGELAAALAGREPPPGLSAEELLSRSWWSGPEIFLIVDDIQQLPPGFDSPLHKAVPFVNRAADVGLHVIVTRTFGGWSSAGSDPMLRALHQANAPLLVMDADPDEGFIRGKMKGGPLPRGRGLLMAEDTGVFVQVAATEVRR