moxR1 Family assigned · medium auto-curated
H37Rv Rv1479 · MTBC0 - ·
377 aa · 1669283–1670416 (+) ·
RefSeq YP_177816.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transcriptional regulator MoxR1 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Transcriptional regulator MoxR1. Pfam: bpMoxR (PF20030.6), AAA_3 (PF07726.18), AAA_5 (PF07728.21), AAA_lid_2 (PF17863.8). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
Q79FN7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Chaperone MoxR1 |
| Curated function | Displays ATP-enhanced chaperone activity. Required for the proper folding of the peptidoglycan endopeptidase RipA and its secretion through the TAT secretion system. In vitro, prevents thermal aggregation of MalZ protein and protects the functional activity of the restriction enzyme NdeI from thermal inactivation..; FUNCTION: Could be a moonlighting protein that uses a multipronged approach to dampen host-directed immunity for efficient replication, survival and pathogenesis. Can enhance virulence by inhibiting autophagy and apoptosis, and disrupting cellular bioenergetics. Binds and activates. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | moxR1 |
| eggNOG description | associated with various cellular activities |
| Orthologous group | COG0714 |
| KEGG orthology |
K03924
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.236 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
bpMoxR | PF20030.6 | 8.6e-15 | 43–218 | MoxR domain in the MoxR-vWA-beta-propeller ternary systems |
AAA_3 | PF07726.18 | 2.3e-60 | 73–203 | ATPase family associated with various cellular activities (AAA) |
AAA_5 | PF07728.21 | 1.7e-15 | 73–201 | AAA domain (dynein-related subfamily) |
AAA_lid_2 | PF17863.8 | 2.2e-17 | 279–341 | AAA lid domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1481 (membrane protein), high confidence from genomic context alone (score 969 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1480 hyp |
hypothetical protein | 986 | 984 ctx | neighborhood:882 cooccurence:742 coexpression:414 |
Rv1481 |
membrane protein | 990 | 969 ctx | neighborhood:874 cooccurence:608 coexpression:426 textmining:707 |
Rv1478 ripB |
peptidoglycan endopeptidase RipB | 813 | 736 ctx | neighborhood:728 |
Rv1483 fabG1 |
3-oxoacyl-ACP reductase FabG | 557 | 557 ctx | neighborhood:544 |
Rv1484 inhA |
NADH-dependent enoyl-[ACP | 554 | 555 ctx | neighborhood:544 |
Rv1485 hemZ |
ferrochelatase | 726 | 547 ctx | neighborhood:544 textmining:422 |
Rv1477 ripA |
peptidoglycan endopeptidase RipA | 839 | 528 ctx | neighborhood:504 textmining:674 |
Rv3163c hyp |
hypothetical protein | 454 | 428 | |
Rv2332 mez |
malate oxidoreductase | 425 | 425 | coexpression:415 |
Rv2531c |
amino acid decarboxylase | 438 | 414 | experimental:405 |
Rv1837c glcB |
malate synthase | 410 | 411 | coexpression:411 |
Rv3596c clpC1 |
ATP-dependent protease ATP-binding subunit ClpC | 414 | 391 | |
Rv2903c lepB |
signal peptidase | 488 | 137 | textmining:432 |
Rv3045 adhC |
NADP-dependent alcohol dehydrogenase | 459 | 91 | textmining:430 |
Rv2888c amiC |
amidase AmiC | 474 | 76 | textmining:455 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): transcriptional regulator MoxR1
- Pfam (hmmscan --cut_ga): bpMoxR PF20030.6 (E=9e-15), AAA_3 PF07726.18 (E=2e-60), AAA_5 PF07728.21 (E=2e-15), AAA_lid_2 PF17863.8 (E=2e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177816.1)
- Domains: Pfam-A via hmmscan --cut_ga — bpMoxR (PF20030.6), AAA_3 (PF07726.18), AAA_5 (PF07728.21), AAA_lid_2 (PF17863.8)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0714 - Curated reference: UniProt Q79FN7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
17 functional partner(s); context anchor
Rv1481 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1479|moxR1 MTSAGGFPAGAGGYQTPGGHSASPAHEAPPGGAEGLAAEVHTLERAIFEVKRIIVGQDQLVERMLVGLLSKGHVLLEGVPGVAKTLAVETFARVVGGTFSRIQFTPDLVPTDIIGTRIYRQGREEFDTELGPVVANFLLADEINRAPAKVQSALLEVMQERHVSIGGRTFPMPSPFLVMATQNPIEHEGVYPLPEAQRDRFLFKINVGYPSPEEEREIIYRMGVTPPQAKQILSTGDLLRLQEIAANNFVHHALVDYVVRVVFATRKPEQLGMNDVKSWVAFGASPRASLGIIAAARSLALVRGRDYVIPQDVIEVIPDVLRHRLVLTYDALADEISPEIVINRVLQTVALPQVNAVPQQGHSVPPVMQAAAAASGR