Rv1481 Family assigned · medium auto-curated
H37Rv Rv1481 · MTBC0 mtbc0_001584 ·
335 aa · 1681182–1682189 (+) ·
RefSeq NP_215997.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | VWA domain-containing protein |
| Revised (this work) | VWA domain-containing protein. Pfam: BatA (PF07584.17), VWA_2 (PF13519.13), VWA (PF00092.35). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WFJ7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UPF0353 protein Rv1481 |
UniProt still lists this protein as UPF0353 protein Rv1481; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | von Willebrand factor, type A |
| Orthologous group | COG2304 |
| KEGG orthology |
K07114
|
| Gene Ontology (12) |
GO:0005575, GO:0005623, GO:0005886, GO:0005887, GO:0016020, GO:0016021, GO:0031224, GO:0031226, GO:0044425, GO:0044459, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.214 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
BatA | PF07584.17 | 1.5e-05 | 12–86 | Aerotolerance regulator N-terminal |
VWA_2 | PF13519.13 | 1.5e-20 | 99–212 | von Willebrand factor type A domain |
VWA | PF00092.35 | 1.3e-13 | 99–288 | von Willebrand factor type A domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: moxR1 (transcriptional regulator MoxR1), high confidence from genomic context alone (score 969 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1480 hyp |
hypothetical protein | 994 | 994 ctx | neighborhood:874 cooccurence:774 coexpression:819 |
Rv1479 moxR1 |
transcriptional regulator MoxR1 | 990 | 969 ctx | neighborhood:874 cooccurence:608 coexpression:426 textmining:707 |
Rv0958 |
magnesium chelatase | 713 | 695 | experimental:617 |
Rv2850c |
magnesium chelatase | 690 | 670 | experimental:617 |
Rv2902c rnhB |
ribonuclease HII | 604 | 605 | coexpression:553 |
Rv1478 ripB |
peptidoglycan endopeptidase RipB | 916 | 594 ctx | neighborhood:575 textmining:803 |
Rv1477 ripA |
peptidoglycan endopeptidase RipA | 805 | 585 ctx | neighborhood:566 textmining:549 |
Rv0049 hyp |
hypothetical protein | 581 | 582 ctx | cooccurence:579 |
Rv3753c hyp |
hypothetical protein | 568 | 569 ctx | cooccurence:566 |
Rv1485 hemZ |
ferrochelatase | 591 | 550 ctx | neighborhood:544 |
Rv1484 inhA |
NADH-dependent enoyl-[ACP | 549 | 549 ctx | neighborhood:544 |
Rv1483 fabG1 |
3-oxoacyl-ACP reductase FabG | 548 | 548 ctx | neighborhood:544 |
Rv3013 hyp |
hypothetical protein | 538 | 538 ctx | cooccurence:528 |
Rv2468c hyp |
hypothetical protein | 502 | 503 ctx | cooccurence:498 |
Rv1638A hyp |
hypothetical protein | 451 | 451 ctx | cooccurence:445 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: membrane protein
- MTBC0 PGAP product: VWA domain-containing protein
- Pfam (hmmscan --cut_ga): BatA PF07584.17 (E=2e-05), VWA_2 PF13519.13 (E=2e-20), VWA PF00092.35 (E=1e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215997.1)
- Domains: Pfam-A via hmmscan --cut_ga — BatA (PF07584.17), VWA_2 (PF13519.13), VWA (PF00092.35)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2304 - Curated reference: UniProt P9WFJ7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
25 functional partner(s); context anchor
moxR1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001584|Rv1481| MTLPLLGPMTLSGFAHSWFFLFLFVVAGLVALYILMQLARQRRMLRFANMELLESVAPKRPSRWRHVPAILLVLSLLLFTIAMAGPTHDVRIPRNRAVVMLVIDVSQSMRATDVEPSRMVAAQEAAKQFADELTPGINLGLIAYAGTATVLVSPTTNREATKNALDKLQFADRTATGEAIFTALQAIATVGAVIGGGDTPPPARIVLFSDGKETMPTNPDNPKGAYTAARTAKDQGVPISTISFGTPYGFVEINDQRQPVPVDDETMKKVAQLSGGNSYNAATLAELRAVYSSLQQQIGYETIKGDASVGWLRLGALALALAALAALLINRRLPT