mbtL Resolved · high auto-curated
H37Rv Rv1344 · MTBC0 - ·
106 aa · 1508968–1509288 (+) ·
RefSeq NP_215860.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyl carrier protein MbtL |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Acyl carrier protein MbtL. Pfam: PP-binding (PF00550.32). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WQF1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acyl carrier protein MbtL |
| Curated function | Acyl carrier protein involved in the formation of acyl-S-ACP intermediates within the mycobactin biosynthesis process. The aliphatic chains carried by ACP are subsequently transferred on to the mycobactin core by MbtK. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| Preferred name | mbtL |
| eggNOG description | acyl carrier protein |
| Orthologous group | COG0236 |
| KEGG orthology |
K02078
|
| Gene Ontology (89) |
GO:0000035, GO:0000036, GO:0003674, GO:0005488, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005975, GO:0006082, GO:0006518 +77 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.239 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PP-binding | PF00550.32 | 2.1e-13 | 33–99 | Phosphopantetheine attachment site |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mbtM (long-chain-fatty-acid--ACP ligase MbtM), high confidence from genomic context alone (score 928 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2524c fas |
fatty acid synthase | 987 | 984 | coexpression:646 experimental:895 database:549 |
Rv1345 mbtM |
long-chain-fatty-acid--ACP ligase MbtM | 964 | 928 ctx | neighborhood:882 textmining:532 |
Rv1346 mbtN |
acyl-[acyl-carrier-protein | 918 | 918 ctx | neighborhood:882 |
Rv2244 acpM |
meromycolate extension acyl carrier protein | 908 | 900 | database:900 |
Rv3147 nuoC |
NADH-quinone oxidoreductase subunit C | 855 | 850 | coexpression:424 experimental:449 database:564 |
Rv3146 nuoB |
NADH-quinone oxidoreductase subunit B | 850 | 844 | coexpression:402 experimental:449 database:564 |
Rv3153 nuoI |
NADH-quinone oxidoreductase subunit I | 850 | 843 | experimental:449 database:564 |
Rv2243 fabD |
malonyl CoA-acyl carrier protein transacylase | 844 | 837 | coexpression:409 experimental:422 database:549 |
Rv3149 nuoE |
NADH-quinone oxidoreductase subunit E | 834 | 830 | coexpression:407 experimental:449 database:518 |
Rv0310c hyp |
hypothetical protein | 816 | 808 | experimental:449 database:561 |
Rv3148 nuoD |
NADH-quinone oxidoreductase subunit D | 790 | 781 | experimental:449 database:564 |
Rv2383c mbtB |
phenyloxazoline synthase | 868 | 779 | coexpression:674 textmining:431 |
Rv3145 nuoA |
NADH-quinone oxidoreductase subunit A | 759 | 759 | experimental:449 database:561 |
Rv3157 nuoM |
NADH-quinone oxidoreductase subunit M | 753 | 753 | experimental:449 database:561 |
Rv3152 nuoH |
NADH-quinone oxidoreductase subunit H | 748 | 749 | experimental:449 database:561 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): acyl carrier protein MbtL
- Pfam (hmmscan --cut_ga): PP-binding PF00550.32 (E=2e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215860.1)
- Domains: Pfam-A via hmmscan --cut_ga — PP-binding (PF00550.32)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0236 - Curated reference: UniProt P9WQF1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
157 functional partner(s); context anchor
mbtM - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1344|mbtL MWRYPLSTRLALPNTPGVASFAMTSSPSTVSTTLLSILRDDLNIDLTRVTPDARLVDDVGLDSVAFAVGMVAIEERLGVALSEEELLTCDTVGELEAAIAAKYRDE