Rv1120c Family assigned · medium auto-curated
H37Rv Rv1120c · MTBC0 - ·
164 aa · 1243010–1243504 (-) ·
RefSeq NP_215636.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Contains Guanylate_cyc (PF00211.26) domain(s); putative function inferred from the domain architecture. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O06572
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | Guanylate cyclase domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
T Signal transduction mechanisms
|
|---|---|
| eggNOG description | PFAM Adenylyl cyclase class-3 4 guanylyl cyclase |
| Orthologous group | COG2114 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.014 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Guanylate_cyc | PF00211.26 | 3.1e-08 | 52–151 | Adenylate and Guanylate cyclase catalytic domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1359 (transcriptional regulator), medium confidence from genomic context alone (score 574 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1119c hyp |
hypothetical protein | 882 | 882 ctx | neighborhood:882 |
Rv1118c hyp |
hypothetical protein | 743 | 743 ctx | neighborhood:710 |
Rv0891c hyp |
hypothetical protein | 699 | 699 ctx | cooccurence:699 |
Rv1359 |
transcriptional regulator | 574 | 574 ctx | cooccurence:574 |
Rv1121 zwf1 |
glucose-6-phosphate 1-dehydrogenase | 557 | 556 ctx | neighborhood:552 |
Rv1122 gnd2 |
6-phosphogluconate dehydrogenase (decarboxylating) | 519 | 519 ctx | neighborhood:519 |
Rv0902c prrB |
two component sensor histidine kinase PrrB | 531 | 517 ctx | cooccurence:446 |
Rv2434c |
transmembrane protein | 515 | 500 | |
Rv1780 hyp |
hypothetical protein | 460 | 460 ctx | cooccurence:460 |
Rv3371 |
diacyglycerol O-acyltransferase | 428 | 429 ctx | cooccurence:427 |
Rv3091 hyp |
hypothetical protein | 420 | 420 ctx | cooccurence:420 |
Rv3239c |
transmembrane transport protein | 437 | 415 | |
Rv3728 |
membrane protein | 434 | 411 | |
Rv2564 glnQ |
glutamine ABC transporter ATP-binding protein | 432 | 411 | |
Rv0073 |
glutamine ABC transporter ATP-binding protein | 430 | 408 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): hypothetical protein
- Pfam (hmmscan --cut_ga): Guanylate_cyc PF00211.26 (E=3e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215636.1)
- Domains: Pfam-A via hmmscan --cut_ga — Guanylate_cyc (PF00211.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2114 - Curated reference: UniProt O06572 (TrEMBL, unreviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
20 functional partner(s); context anchor
Rv1359 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1120c| MLSGGREAVKTVWQTANLVRKEGFGAAVRSSIEDPADWAEVERPDLARVTPDGRVVILFSDIEESTALDERIGDRTWVKLIGAHDKLVHELVRRWSGHMVTSQGDGFMIAFARAEQAVRCGIDIQDALRNSAKRKRNQGIRVRIGTTWGARCGTVTICSAATSQ