zwf1 Resolved · high auto-curated
H37Rv Rv1121 · MTBC0 - ·
466 aa · 1243707–1245107 (+) ·
RefSeq YP_177789.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | glucose-6-phosphate 1-dehydrogenase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Glucose-6-phosphate 1-dehydrogenase. Pfam: G6PD_N (PF00479.29), G6PD_C (PF02781.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WN71
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Glucose-6-phosphate 1-dehydrogenase 1 |
| EC (curated) |
EC 1.1.1.49
|
| Curated function | Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | zwf1 |
| eggNOG description | Catalyzes the oxidation of glucose 6-phosphate to 6- phosphogluconolactone |
| Orthologous group | COG0364 |
| EC number |
EC 1.1.1.363, EC 1.1.1.49
|
| KEGG orthology |
K00036
|
| KEGG pathways |
map00030, map00480, map01100, map01110, map01120, map01130, map01200, map05230
|
| KEGG modules |
M00004, M00006, M00008
|
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.677 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 7 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.17% of strains (252) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
G6PD_N | PF00479.29 | 2.6e-40 | 13–178 | Glucose-6-phosphate dehydrogenase, NAD binding domain |
G6PD_C | PF02781.22 | 9.5e-88 | 183–452 | Glucose-6-phosphate dehydrogenase, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: devB (6-phosphogluconolactonase), high confidence from genomic context alone (score 998 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1445c devB |
6-phosphogluconolactonase | 999 | 998 ctx | fusion:827 cooccurence:755 coexpression:482 database:900 textmining:749 |
Rv1122 gnd2 |
6-phosphogluconate dehydrogenase (decarboxylating) | 984 | 980 ctx | neighborhood:851 fusion:454 cooccurence:754 |
Rv0946c pgi |
glucose-6-phosphate isomerase | 974 | 952 | coexpression:482 database:900 textmining:497 |
Rv1844c gnd1 |
6-phosphogluconate dehydrogenase | 973 | 928 ctx | cooccurence:733 coexpression:658 textmining:650 |
Rv1447c zwf2 |
glucose-6-phosphate 1-dehydrogenase | 926 | 916 | database:900 |
Rv1436 gap |
glyceraldehyde 3-phosphate dehydrogenase | 951 | 899 | coexpression:436 database:800 textmining:540 |
Rv3068c pgmA |
phosphoglucomutase PgmA | 921 | 890 | database:800 |
Rv1617 pykA |
pyruvate kinase | 899 | 847 | database:800 |
Rv0489 gpm1 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 851 | 832 | database:800 |
Rv1438 tpi |
triosephosphate isomerase | 871 | 818 | database:800 |
Rv0363c fba |
fructose-bisphosphate aldolase | 836 | 815 | database:800 |
Rv2702 ppgK |
polyphosphate glucokinase | 862 | 812 | database:800 |
Rv0650 |
sugar kinase | 895 | 811 | database:800 textmining:473 |
Rv2241 aceE |
pyruvate dehydrogenase E1 component | 830 | 809 | database:800 |
Rv2455c korA |
2-oxoglutarate oxidoreductase subunit KorA | 807 | 808 | database:800 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): glucose-6-phosphate 1-dehydrogenase
- Pfam (hmmscan --cut_ga): G6PD_N PF00479.29 (E=3e-40), G6PD_C PF02781.22 (E=9e-88)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177789.1)
- Domains: Pfam-A via hmmscan --cut_ga — G6PD_N (PF00479.29), G6PD_C (PF02781.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0364 - Curated reference: UniProt P9WN71 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
49 functional partner(s); context anchor
devB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1121|zwf1 MVDGGGGASDLLVIFGITGDLARKMTFRALYRLERHQLLDCPILGVASDDMSVGQLVKWARESIGRTEKIDDAVFDRLAGRLSYLHGDVTDSQLYDSLAELIGSACRPLYYLEMPPALFAPIVENLANVRLLERARVAVEKPFGHDLASALELNARLRAVLGEDQILRVDHFLGKQPVVELEYLRFANQALAELWDRNSISEIHITMAEDFGVEDRGKFYDAVGALRDVVQNHLLQVLALVTMEPPVGSSADDLNDKKAEVFRAMAPLDPDRCVRGQYLGYTEVAGVASDSATETYVALRTEIDNWRWAGVPIFVRAGKELPAKVTEVRLFLRRVPALAFLPNRRPAEPNQIVLRIDPDPGMRLQISAHTDDSWRDIHLDSSFAVDLGEPIRPYERLLYAGLVGDHQLFAREDSIEQTWRIVQPLLDNPGEIHRYDRGSWGPEAAQSLLRGHRGWQSPWLPRGTDA