Rv0730 Resolved · high auto-curated
H37Rv Rv0730 · MTBC0 - ·
242 aa · 822866–823594 (+) ·
RefSeq NP_215244.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | GCN5-like N-acetyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | GCN5-like N-acetyltransferase. Pfam: Acetyltransf_3 (PF13302.14), Acetyltransf_1 (PF00583.32). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
I6XW38
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | GCN5-related N-acetyltransferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| eggNOG description | Type IIA topoisomerase (DNA gyrase topo II, topoisomerase IV), A subunit |
| Orthologous group | COG0188 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 3 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.12% of strains (176) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acetyltransf_3 | PF13302.14 | 3.4e-06 | 114–213 | Acetyltransferase (GNAT) domain |
Acetyltransf_1 | PF00583.32 | 1.4e-05 | 159–211 | Acetyltransferase (GNAT) family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: xylB (D-xylulose kinase XylB), high confidence from genomic context alone (score 858 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0005 gyrB |
DNA gyrase subunit B | 956 | 951 | coexpression:726 experimental:818 |
Rv0729 xylB |
D-xylulose kinase XylB | 981 | 858 ctx | neighborhood:858 textmining:875 |
Rv1307 atpH |
ATP synthase subunit b/delta | 679 | 679 | coexpression:655 |
Rv2089c pepE |
dipeptidase PepE | 627 | 605 ctx | neighborhood:544 |
Rv0728c serA2 |
D-3-phosphoglycerate dehydrogenase SerA | 605 | 605 ctx | neighborhood:601 |
Rv0727c fucA |
L-fuculose phosphate aldolase FucA | 619 | 603 ctx | neighborhood:601 |
Rv0705 rpsS |
30S ribosomal protein S19 | 579 | 558 | |
Rv1650 pheT |
phenylalanine--tRNA ligase subunit beta | 629 | 514 | coexpression:428 |
Rv2841c nusA |
transcription termination/antitermination protein NusA | 537 | 493 | coexpression:477 |
Rv1407 fmu |
16S rRNA m5C967 methyltransferase | 522 | 488 | coexpression:418 |
Rv1640c lysX |
bifunctional lysine--tRNA ligase/phosphatidylglycerol lysyltransferase | 612 | 467 | |
Rv3598c lysS |
lysine--tRNA ligase | 503 | 461 | |
Rv2903c lepB |
signal peptidase | 479 | 447 | coexpression:415 |
Rv0685 tuf |
elongation factor Tu | 509 | 444 | coexpression:443 |
Rv1649 pheS |
phenylalanine--tRNA ligase subunit alpha | 509 | 443 | coexpression:411 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): GCN5-like N-acetyltransferase
- Pfam (hmmscan --cut_ga): Acetyltransf_3 PF13302.14 (E=3e-06), Acetyltransf_1 PF00583.32 (E=1e-05)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215244.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acetyltransf_3 (PF13302.14), Acetyltransf_1 (PF00583.32)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0188 - Curated reference: UniProt I6XW38 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
66 functional partner(s); context anchor
xylB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0730| MHGARTGVSFYAYAMTDHDQTAARREIADALLAALERRHEVADAIVEAANKAAAVEAIVNLLGTSHLAAEAVMSMSFDQLTQDARTKIIAELDDLNKQLSFTVKERPASSGEGLELRPFSPDEDRDIFARRTEEMGAAGDGSGGPAGSVDDEIRAAQKRVDDEEAAWFVAVDSGVKVGMVFGELVHGEVDVRIWIHPDHRKKGYGTAALRKSRSEMAWAFPAVPMVARAPAAQPAQPGSAGR