murJ Resolved · high auto-curated
H37Rv Rv3910 · MTBC0 mtbc0_004144 ·
1184 aa · 4420849–4424403 (+) ·
RefSeq NP_218427.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | peptidoglycan biosynthesis protein |
|---|---|
| MTBC0 PGAP re-annotation | murein biosynthesis integral membrane protein MurJ |
| Revised (this work) | Murein biosynthesis integral membrane protein MurJ. Pfam: MurJ (PF03023.20). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJK3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable peptidoglycan biosynthesis protein MviN |
| Curated function | Essential for cell growth and peptidoglycan synthesis. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mviN |
| eggNOG description | Integral membrane protein MviN |
| Orthologous group | COG0728 |
| KEGG orthology |
K03980
|
| Gene Ontology (30) |
GO:0003674, GO:0005215, GO:0005575, GO:0005576, GO:0005623, GO:0005886, GO:0005887, GO:0006810, GO:0006855, GO:0008150, GO:0015238, GO:0015893 +18 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.443 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 28 synonymous, 32 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MurJ | PF03023.20 | 1.8e-126 | 48–521 | Lipid II flippase MurJ |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fhaA (FHA domain-containing protein FhaA), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0020c fhaA |
FHA domain-containing protein FhaA | 999 | 1000 ctx | cooccurence:643 experimental:999 textmining:914 |
Rv3909 hyp |
hypothetical protein | 953 | 934 ctx | neighborhood:881 cooccurence:446 |
Rv3908 mutT4 |
mutator protein MutT | 884 | 884 ctx | neighborhood:881 |
Rv3906c hyp |
hypothetical protein | 830 | 830 ctx | neighborhood:618 cooccurence:569 |
Rv3912 rsmA |
anti-sigma-M factor RsmA | 816 | 816 ctx | neighborhood:815 |
Rv3911 sigM |
ECF RNA polymerase sigma factor SigM | 746 | 737 ctx | neighborhood:724 |
Rv3850 hyp |
hypothetical protein | 717 | 717 ctx | cooccurence:713 |
Rv1125 hyp |
hypothetical protein | 709 | 710 ctx | cooccurence:709 |
Rv3244c lpqB |
lipoprotein LpqB | 692 | 692 ctx | cooccurence:666 |
Rv3805c aftB |
terminal beta-(1->2)-arabinofuranosyltransferase | 685 | 686 ctx | cooccurence:608 |
Rv1610 |
membrane protein | 682 | 683 ctx | cooccurence:678 |
Rv0475 hbhA |
heparin binding hemagglutinin HbhA | 682 | 683 ctx | cooccurence:681 |
Rv3907c pcnA |
poly(A) polymerase PcnA | 680 | 680 ctx | neighborhood:679 |
Rv2342 hyp |
hypothetical protein | 675 | 675 ctx | cooccurence:673 |
Rv0383c ttfA hyp |
hypothetical protein | 671 | 671 ctx | cooccurence:661 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: peptidoglycan biosynthesis protein
- MTBC0 PGAP product: murein biosynthesis integral membrane protein MurJ
- Pfam (hmmscan --cut_ga): MurJ PF03023.20 (E=2e-126)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218427.1)
- Domains: Pfam-A via hmmscan --cut_ga — MurJ (PF03023.20)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0728 - Curated reference: UniProt P9WJK3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
108 functional partner(s); context anchor
fhaA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_004144|Rv3910|murJ MRPSPGEVPTASQRQPELSDAALVSHSWAMAFATLISRITGFARIVLLAAILGAALASSFSVANQLPNLVAALVLEATFTAIFVPVLARAEQDDPDGGAAFVRRLVTLATTLLLGATTLSVLAAPLLVRLMLGTNPQVNEPLTTAFAYLLLPQVLVYGLSSVFMAILNTRNVFGPPAWAPVVNNVVAIATLAVYLAVPGELSVDPVRMGNAKLLVLGIGTTAGVFAQTAVLLVAIRREHISLRPLWGIDQRLKRFGAMAAAMVLYVLISQLGLVVGNRIASTAAASGPAIYNYTWLVLMLPFGMIGVTVLTVVMPRLSRNAAADDTPAVLADLSLATRLTMITLIPTVAFMTVGGPAIGSALFAYGNFGDVDAGYLGAAIALSAFTLIPYALVLLQLRVFYAREQPWTPITIIVVITGVKILGSLLAPHITGDPQLVAAYLGLANGLGFLAGTIVGYYILRRALRPDGGQLIGVGEARTVLVTVAASLLAGLLAHVADRLLGLSELTAHAGSVGSLLRLSVLALIMLPILAAVTLCARVPEARAALDAVRARIRSRRLKTGPQTQNVLDQSSRPGPVTYPERRRLAPPRGKSVVHEPIRRRPPEQVARAGRAKGPEVIDRPSENASFGAASGAELPRPVADELQLDAPAGRDPGPVSRPHPSDLQNGDLPADAARGPIAFDALREPDRESSAPPDDVQLVPGARIANGRYRLLIFHGGVPPLQFWQALDTALDRQVALTFVDPQGVLPDDVLQETLSRTLRLSRIDKPGVARVLDVVHTRAGGLVVAEWIRGGSLQEVADTSPSPVGAIRAMQSLAAAADAAHRAGVALSIDHPSRVRVSIDGDVVLAYPATMPDANPQDDIRGIGASLYALLVNRWPLPEAGVRSGLAPAERDTAGQPIEPADIDRDIPFQISAVAARSVQGDGGIRSASTLLNLMQQATAVADRTEVLGPIDEAPVSAAPRTSAPNSETYTRRRRNLLIGIGAGAAVLMVALLVLASVLSRIFGDVSGGLNKDELGLNAPTASTSAASSAPPGSVVKPTKVTVFSPDGGADNPGEADLAIDGNPATSWKTDIYTDPVPFPSFKNGVGLMLQLPQATVVGTVAIDVASTGTKVEIRSASTPTPATLEDTAVLTSATALRPGHNTISVEAAAPTSNLLVWISTLGTTDGKSQADISEITIYAAS