Rv3591c Family assigned · medium auto-curated
H37Rv Rv3591c · MTBC0 mtbc0_003810 ·
257 aa · 4056946–4057719 (-) ·
RefSeq NP_218108.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hydrolase |
|---|---|
| MTBC0 PGAP re-annotation | alpha/beta hydrolase |
| Revised (this work) | Alpha/beta hydrolase. Pfam: Abhydrolase_1 (PF00561.27), Abhydrolase_6 (PF12697.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6YGL1
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible hydrolase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Alpha beta hydrolase |
| Orthologous group | COG2267 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.702 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Abhydrolase_1 | PF00561.27 | 3.6e-09 | 18–115 | alpha/beta hydrolase fold |
Abhydrolase_6 | PF12697.14 | 1.4e-17 | 19–245 | Alpha/beta hydrolase family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mhuD (heme-degrading monooxygenase), high confidence from genomic context alone (score 791 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3592 mhuD |
heme-degrading monooxygenase | 791 | 791 ctx | neighborhood:791 |
Rv3593 lpqF |
lipoprotein LpqF | 790 | 791 ctx | neighborhood:791 |
Rv3203 lipV |
lipase LipV | 626 | 627 ctx | cooccurence:625 |
Rv2418c octT hyp |
hypothetical protein | 632 | 619 ctx | cooccurence:609 |
Rv3590c PE_PGRS58 |
PE-PGRS family protein PE_PGRS58 | 562 | 562 ctx | neighborhood:562 |
Rv3415c hyp |
hypothetical protein | 541 | 542 ctx | cooccurence:537 |
Rv1527c pks5 |
polyketide synthase | 533 | 506 | experimental:441 |
Rv2048c pks12 |
polyketide synthase | 527 | 500 | experimental:441 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 526 | 498 | experimental:441 |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 526 | 498 | experimental:441 |
Rv2933 ppsC |
phthiocerol synthesis polyketide synthase type I PpsC | 526 | 498 | experimental:441 |
Rv2695 hyp |
hypothetical protein | 483 | 483 ctx | cooccurence:478 |
Rv2186c hyp |
hypothetical protein | 463 | 464 ctx | cooccurence:462 |
Rv2946c pks1 |
polyketide synthase | 487 | 457 | |
Rv3355c |
integral membrane protein | 432 | 432 ctx | cooccurence:432 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hydrolase
- MTBC0 PGAP product: alpha/beta hydrolase
- Pfam (hmmscan --cut_ga): Abhydrolase_1 PF00561.27 (E=4e-09), Abhydrolase_6 PF12697.14 (E=1e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218108.1)
- Domains: Pfam-A via hmmscan --cut_ga — Abhydrolase_1 (PF00561.27), Abhydrolase_6 (PF12697.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2267 - Curated reference: UniProt I6YGL1 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
20 functional partner(s); context anchor
mhuD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003810|Rv3591c| MPRMPANLLTHRGGRGEPLVLVHGLMGRGSTWARQLPWLTLLGAVYTYDAPWHRGRDVADPHPISTERFVADLGDAVSALGAPTRMVGHSMGALHSWCLAAERPELVSALVVEDMAPDFRGRTTGPWEPWLRALPVEFDSAEQVFAEFGPVAGRYFLDAFDRTATGWRLHGRTARWIEIAAEWGTRDYWAQWRAVRSPALLIEAGDGVTPPGQMRAMAERDYPTAYLRVPDAGHLVHDEAPQVYRRAVESFLAGLTP