iunH Resolved · high auto-curated
H37Rv Rv3393 · MTBC0 mtbc0_003605 ·
308 aa · 3833900–3834826 (+) ·
RefSeq NP_217910.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | nucleoside hydrolase |
|---|---|
| MTBC0 PGAP re-annotation | nucleoside hydrolase IunH |
| Revised (this work) | Nucleoside hydrolase IunH. Pfam: IU_nuc_hydro (PF01156.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O50418
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable nucleoside hydrolase IunH |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
F Nucleotide transport and metabolism
|
|---|---|
| Preferred name | iunH |
| eggNOG description | nucleoside hydrolase |
| Orthologous group | COG1957 |
| EC number |
EC 3.2.2.1
|
| KEGG orthology |
K01239, K01250
|
| KEGG pathways |
map00230, map00760, map01100
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.445 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
IU_nuc_hydro | PF01156.25 | 7.1e-79 | 4–295 | Inosine-uridine preferring nucleoside hydrolase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: rbsK (ribokinase RbsK), high confidence from genomic context alone (score 794 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3307 deoD |
purine nucleoside phosphorylase | 963 | 917 | database:900 textmining:583 |
Rv2202c adoK |
adenosine kinase | 925 | 915 | database:900 |
Rv2043c pncA |
pyrazinamidase/nicotinamidase PncA | 928 | 913 | database:900 |
Rv3313c add |
adenosine deaminase | 918 | 910 | database:900 |
Rv1151c cobB |
NAD-dependent protein deacylase | 909 | 904 | database:900 |
Rv2584c apt |
adenine phosphoribosyltransferase | 982 | 903 | database:900 textmining:832 |
Rv3624c hpt |
hypoxanthine-guanine phosphoribosyltransferase | 967 | 901 | database:900 textmining:688 |
Rv0212c nadR |
transcriptional regulator NadR | 903 | 900 | database:900 |
Rv2436 rbsK |
ribokinase RbsK | 887 | 794 ctx | cooccurence:726 textmining:478 |
Rv3315c cdd |
cytidine deaminase | 620 | 575 | database:500 |
Rv3392c cmaA1 |
cyclopropane mycolic acid synthase CmaA | 573 | 573 ctx | neighborhood:567 |
Rv3752c tadA |
cytidine/deoxycytidylate deaminase | 575 | 559 | database:500 |
Rv1629 polA |
DNA polymerase I | 528 | 216 | textmining:423 |
Rv0787A purS hyp |
hypothetical protein | 709 | 164 | textmining:667 |
Rv0994 moeA1 |
molybdopterin molybdenumtransferase 1 | 599 | 163 | textmining:541 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: nucleoside hydrolase
- MTBC0 PGAP product: nucleoside hydrolase IunH
- Pfam (hmmscan --cut_ga): IU_nuc_hydro PF01156.25 (E=7e-79)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217910.1)
- Domains: Pfam-A via hmmscan --cut_ga — IU_nuc_hydro (PF01156.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1957 - Curated reference: UniProt O50418 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
37 functional partner(s); context anchor
rbsK - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003605|Rv3393|iunH MSVVFADVDTGIDDALAVIYLLASPDADLVGIASTGGNIAVGQVCANNLSLLELCGAADIPVSKGADEPLGGRWPDHPKFHGPKGIGYAELPASNRRLTDYDATTAWIAAAHSHAGDLIGLVTGPLTNLALALRAEPALPRLLRRLVIMGGMFDGQPITEWNIRVDPEAASEVFTAWAGQRQLPIVCGLDLTRRVAMTPDILARLASVCGSSPVMRVIEDALRFYFESHEARGHGYLAYMHDPLAAAVAMDPELLTTRTATVDVDPTGATVTDWSGKRNPNARIGMSVDPAVFFDRFVERIGRFARRT