Rv3026c Family assigned · medium auto-curated
H37Rv Rv3026c · MTBC0 mtbc0_003217 ·
304 aa · 3406473–3407387 (-) ·
RefSeq NP_217542.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | lysophospholipid acyltransferase family protein |
| Revised (this work) | Lysophospholipid acyltransferase family protein. Pfam: Acyltransferase (PF01553.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6XFY8
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Phospholipid/glycerol acyltransferase domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Acyltransferase |
| Orthologous group | COG0204 |
| EC number |
EC 2.3.1.51
|
| KEGG orthology |
K00655
|
| KEGG pathways |
map00561, map00564, map01100, map01110
|
| KEGG modules |
M00089
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.561 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 10 synonymous, 14 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acyltransferase | PF01553.27 | 5.2e-22 | 81–222 | Acyltransferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3027c (GCN5-like N-acetyltransferase), high confidence from genomic context alone (score 976 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3027c |
GCN5-like N-acetyltransferase | 985 | 976 ctx | neighborhood:882 cooccurence:773 textmining:424 |
Rv3024c trmU |
tRNA-specific 2-thiouridylase | 625 | 625 ctx | neighborhood:623 |
Rv3025c iscS |
cysteine desulfurase | 623 | 623 ctx | neighborhood:623 |
Rv3028c fixB |
electron transfer flavoprotein subunit alpha | 579 | 579 ctx | neighborhood:575 |
Rv3029c fixA |
electron transfer flavoprotein subunit beta | 527 | 528 ctx | neighborhood:520 |
Rv0107c ctpI |
cation-transporter ATPase I | 467 | 466 | database:451 |
Rv0425c ctpH |
metal cation transporting ATPase H | 466 | 466 | database:451 |
Rv1997 ctpF |
cation transporter ATPase F | 466 | 466 | database:451 |
Rv0908 ctpE |
metal cation transporter ATPase E | 466 | 466 | database:451 |
Rv1305 atpE |
ATP synthase subunit C | 447 | 448 | database:434 |
Rv2139 pyrD |
dihydroorotate dehydrogenase | 427 | 427 | coexpression:419 |
Rv0048c |
membrane protein | 420 | 420 ctx | cooccurence:420 |
Rv0436c pssA |
CDP-diacylglycerol--serine O-phosphatidyltransferase | 439 | 404 | |
Rv2524c fas |
fatty acid synthase | 477 | 316 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: lysophospholipid acyltransferase family protein
- Pfam (hmmscan --cut_ga): Acyltransferase PF01553.27 (E=5e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217542.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acyltransferase (PF01553.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0204 - Curated reference: UniProt I6XFY8 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
14 functional partner(s); context anchor
Rv3027c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003217|Rv3026c| MSAPAVTEHSWLPRATCGVSCVSVGDAAQVRRPLVVLRVALRVMLALLLVPGVPLVVMPLPGRTRVQRIYCRLVLRLFGVRITVSGSPVRNLRGVLVVSGHVSWLDVFCIGSVLPGSFVARADMFTGRTIGIVARILKIIPIERASLRRLPGVVDTIARRLRAGQTVVAFPEGTTWCGRPGDDAGRPAARAGAGCSHRGCGAFYPAMFQAAIDAGRPVQPLRLTYHHVDGTVSTAPAFVGDDTLVRSVCRLLTVRRTLAWVRVESLQLPGTDRRNLARRCQSAVLAGALGQSGQRPGRRHVPAT