Rv0519c Family assigned · medium auto-curated
H37Rv Rv0519c · MTBC0 - ·
300 aa · 611172–612074 (-) ·
RefSeq NP_215033.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Membrane protein. Pfam: Esterase (PF00756.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O33364
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acyl-CoA:diacylglycerol acyltransferase |
| EC (curated) |
EC 2.3.1.122, EC 2.3.1.20
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Putative esterase |
| Orthologous group | COG0627 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.28 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Esterase | PF00756.27 | 6.7e-07 | 90–298 | Putative esterase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pks13 (polyketide synthase), medium confidence from genomic context alone (score 650 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2383c mbtB |
phenyloxazoline synthase | 849 | 800 | coexpression:764 |
Rv1084 hyp |
hypothetical protein | 664 | 652 | experimental:652 |
Rv3800c pks13 |
polyketide synthase | 749 | 650 ctx | neighborhood:401 coexpression:440 |
Rv0272c hyp |
hypothetical protein | 615 | 615 ctx | cooccurence:615 |
Rv0521 |
Possible methyltransferase/methylase (fragment); Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possi | 567 | 567 ctx | neighborhood:506 |
Rv2377c mbtH hyp |
hypothetical protein | 580 | 540 | coexpression:413 |
Rv0290 eccD3 |
ESX-3 secretion system protein EccD | 512 | 513 ctx | cooccurence:508 |
Rv0520 |
Possible methyltransferase/methylase (fragment); Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first | 510 | 509 ctx | neighborhood:506 |
Rv0273c |
transcriptional regulator | 499 | 500 ctx | cooccurence:498 |
Rv2384 mbtA |
2,3-dihydroxybenzoate-AMP ligase | 534 | 482 | coexpression:442 |
Rv2380c mbtE |
peptide synthetase | 515 | 472 | coexpression:405 |
Rv1348 irtA |
iron ABC transporter ATP-binding protein/permease IrtA | 538 | 468 | |
Rv2851c |
GCN5-like N-acetyltransferase | 465 | 466 | coexpression:422 |
Rv1571 hyp |
hypothetical protein | 498 | 459 | coexpression:414 |
Rv0138 hyp |
hypothetical protein | 446 | 446 ctx | cooccurence:446 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): membrane protein
- Pfam (hmmscan --cut_ga): Esterase PF00756.27 (E=7e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215033.1)
- Domains: Pfam-A via hmmscan --cut_ga — Esterase (PF00756.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0627 - Curated reference: UniProt O33364 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
42 functional partner(s); context anchor
pks13 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0519c| MLRRGCAGNTDRRGIMTPMADLTRRALLRWGAGAGAGAAGVWAFGALVDPLEPQAAPAPFEPPTAGSSLPTRISGSFISAARGGIKTNWVISMPPGQSGQLRPVIALHGKDGNAGMMLDLGVEQGLARLVKEGKPAFAVVGVDGGNTYWHRRSSGGDSGAMVLDELLPMLTSMGMDTSRVGFLGWSMGGYGALLLGARLGPARTAGICAISPALFTSFTGSTPGAFDSYDDYVQHSVLGLPALNSIPLRVDCGTSDRFYFATRQFVNQLHQPPAGSFSPGGHDASYWREQLPGELAWMAS