Rv1203c Still unknown · low
H37Rv Rv1203c · MTBC0 - ·
194 aa · 1346321–1346905 (-) ·
RefSeq NP_215719.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Conserved hypothetical; function unknown. Structure-based hint rejected: weak fold echo only (HEAT-repeat alpha-solenoid); function unknown. |
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O05304
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | AAA family ATPase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| eggNOG description | luxR family |
| Orthologous group | COG2909 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.372 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Structural neighbours (Foldseek on the ESMFold model, exploratory)
ESMFold model confidence: mean pLDDT 95.2 (very high). A confident model makes the fold comparison meaningful.
Best matches against the PDB, ranked by Foldseek homology probability. A high probability / TM-score suggests a shared fold; unless flagged sig (E < 0.01) these are fold hypotheses, not assignments.
| Target | Prob | TM | E-value | Description |
|---|---|---|---|---|
6x9o-assembly1_B |
1.00 | 0.51 | 1.0e-03 sig | 6x9o-assembly1_B High resolution cryoEM structure of huntingtin in complex with HAP40 |
8sah-assembly1_B |
1.00 | 0.53 | 2.0e-03 sig | 8sah-assembly1_B Huntingtin C-HEAT domain in complex with HAP40 |
8rsv-assembly2_B |
1.00 | 0.81 | 4.0e-02 | 8rsv-assembly2_B Rap from bacteriophage Phi3T in presence of pheromone RRGHTAS |
8cqq-assembly1_A |
1.00 | 0.40 | 7.5e-04 sig | 8cqq-assembly1_A Crystal structure of an 8-repeat consensus TPR superhelix with Copper |
4gyo-assembly2_A |
1.00 | 0.82 | 8.3e-02 | 4gyo-assembly2_A Crystal Structure of Rap Protein Complexed with Competence and Sporulation Factor |
8rsu-assembly1_A |
1.00 | 0.77 | 3.8e-02 | 8rsu-assembly1_A Rap from bacteriophage Phi3T in presence of pheromone RRGHTA |
5a7d-assembly4_D |
1.00 | 0.61 | 3.7e-02 | 5a7d-assembly4_D Tetrameric assembly of LGN with Inscuteable |
4g2v-assembly1_A |
1.00 | 0.60 | 3.3e-02 | 4g2v-assembly1_A Structure complex of LGN binding with FRMPD1 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: lprB (lipoprotein LprB), high confidence from genomic context alone (score 809 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1204c hyp |
hypothetical protein | 990 | 990 ctx | neighborhood:686 fusion:871 cooccurence:770 |
Rv1274 lprB |
lipoprotein LprB | 808 | 809 ctx | cooccurence:758 |
Rv1275 lprC |
lipoprotein LprC | 773 | 773 ctx | cooccurence:728 |
Rv1125 hyp |
hypothetical protein | 756 | 751 ctx | cooccurence:750 |
Rv3035 hyp |
hypothetical protein | 726 | 727 ctx | cooccurence:723 |
Rv2876 |
transmembrane protein | 704 | 704 ctx | cooccurence:703 |
Rv1610 |
membrane protein | 700 | 701 ctx | cooccurence:700 |
Rv0383c ttfA hyp |
hypothetical protein | 666 | 666 ctx | cooccurence:666 |
Rv3446c hyp |
hypothetical protein | 671 | 658 ctx | cooccurence:654 |
Rv1776c |
transcriptional regulator | 658 | 657 ctx | cooccurence:656 |
Rv2482c plsB2 |
glycerol-3-phosphate acyltransferase | 632 | 632 ctx | cooccurence:630 |
Rv3668c |
protease | 621 | 621 ctx | cooccurence:620 |
Rv3802c |
membrane protein | 619 | 619 ctx | cooccurence:614 |
Rv0756c hyp |
hypothetical protein | 616 | 616 ctx | cooccurence:616 |
Rv0941c hyp |
hypothetical protein | 594 | 595 ctx | cooccurence:592 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Structural Foldseek hit not propagated -- weak fold echo only (HEAT-repeat alpha-solenoid); function unknown
- Reviewed against literature (extended structural cross-check, 2026-06-02)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215719.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2909 - Curated reference: UniProt O05304 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Model confidence: ESMFold per-residue pLDDT (mean 95.2, very high)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
86 functional partner(s); context anchor
lprB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1203c| MLLAYVLITKGEFGAAASMLEPAAATLERTGYSWGPLSLMLLATAIAQQGHIAESAKTLQRAEARHGTKSALFAPELGLARAWTRAAAQDMTGAIAAAREAARTAERAGQAAVALCAWHNAVRLGDIRAVDPVTRLAAEIDCTVGNILVKHARGLADGDAAELTAVAEELAGIGMAAAAADATKAAARLGPQQR