galU Resolved · high auto-curated
H37Rv Rv0993 · MTBC0 mtbc0_001064 ·
306 aa · 1116608–1117528 (+) ·
RefSeq NP_215508.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | UTP--glucose-1-phosphate uridylyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | UTP--glucose-1-phosphate uridylyltransferase |
| Revised (this work) | UTP--glucose-1-phosphate uridylyltransferase. Pfam: NTP_transferase (PF00483.30). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O05576
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UTP--glucose-1-phosphate uridylyltransferase |
| EC (curated) |
EC 2.7.7.9
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | galU |
| eggNOG description | UDP-glucose pyrophosphorylase |
| Orthologous group | COG1210 |
| EC number |
EC 2.7.7.9
|
| KEGG orthology |
K00963
|
| KEGG pathways |
map00040, map00052, map00500, map00520, map01100, map01130
|
| KEGG modules |
M00129, M00361, M00362, M00549
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.074 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
NTP_transferase | PF00483.30 | 6.8e-27 | 12–281 | Nucleotidyl transferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: moeA1 (molybdopterin molybdenumtransferase 1), high confidence from genomic context alone (score 798 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0322 udgA |
UDP-glucose 6-dehydrogenase UdgA | 973 | 963 | coexpression:463 database:900 |
Rv3634c galE1 |
UDP-glucose 4-epimerase | 934 | 922 | database:900 |
Rv0334 rmlA |
glucose-1-phosphate thymidylyltransferase | 927 | 915 | database:900 |
Rv3068c pgmA |
phosphoglucomutase PgmA | 934 | 908 | database:900 |
Rv0501 galE2 |
UDP-glucose 4-epimerase GalE | 910 | 905 | database:900 |
Rv3490 otsA |
trehalose-phosphate synthase | 981 | 904 | database:900 textmining:815 |
Rv3032 |
glycogen synthase | 948 | 904 | database:900 textmining:482 |
Rv3468c |
dTDP-glucose 4,6-dehydratase | 909 | 904 | database:900 |
Rv1212c glgA |
capsular glucan synthase | 992 | 903 | database:900 textmining:922 |
Rv1213 glgC |
glucose-1-phosphate adenylyltransferase | 984 | 901 | database:900 textmining:855 |
Rv1328 glgP |
glycogen phosphorylase | 942 | 900 | database:900 textmining:446 |
Rv1781c malQ |
4-alpha-glucanotransferase | 912 | 900 | database:900 |
Rv0618 galTa |
Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly | 910 | 900 | database:900 |
Rv0994 moeA1 |
molybdopterin molybdenumtransferase 1 | 798 | 798 ctx | neighborhood:787 |
Rv0992c |
5-formyltetrahydrofolate cyclo-ligase | 792 | 781 ctx | neighborhood:781 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: UTP--glucose-1-phosphate uridylyltransferase
- MTBC0 PGAP product: UTP--glucose-1-phosphate uridylyltransferase
- Pfam (hmmscan --cut_ga): NTP_transferase PF00483.30 (E=7e-27)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215508.1)
- Domains: Pfam-A via hmmscan --cut_ga — NTP_transferase (PF00483.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1210 - Curated reference: UniProt O05576 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
47 functional partner(s); context anchor
moeA1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001064|Rv0993|galU MSRPEVLTPFTAIVPAAGLGTRFLPATKTVPKELLPVVDTPGIELVAAEAAAAGAERLVIVTSEGKDGVVAHFVEDLVLEGTLEARGKIAMLAKVRRAPALIKVESVVQAEPLGLGHAIGCVEPTLSPDEDAVAVLLPDDLVLPTGVLETMSKVRASRGGTVLCAIEVAREEISAYGVFDVEPVPDGDYTDDPNVLKVRGMVEKPKAETAPSRYAAAGRYVLDRAIFDALRRIDRGAGGEVQLTDAIALLIAEGHPVHVVVHQGSRHDLGNPGGYLKAAVDFALDRDDYGPDLRRWLVARLGLTEQ