Rv0998 Family assigned · medium auto-curated
H37Rv Rv0998 · MTBC0 mtbc0_001072 ·
333 aa · 1122084–1123085 (+) ·
RefSeq NP_215513.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acetyltransferase Pat |
|---|---|
| MTBC0 PGAP re-annotation | GNAT family N-acetyltransferase |
| Revised (this work) | GNAT family N-acetyltransferase. Pfam: cNMP_binding (PF00027.36), Acetyltransf_3 (PF13302.14), Acetyltransf_1 (PF00583.32). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O05581
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acetyltransferase Pat |
| EC (curated) |
EC 2.3.1.-
|
| Curated function | Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS). This acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesisT Signal transduction mechanisms
|
|---|---|
| eggNOG description | cyclic nucleotide-binding |
| Orthologous group | COG0664 |
| Gene Ontology (24) |
GO:0000166, GO:0003674, GO:0003824, GO:0005488, GO:0008080, GO:0016407, GO:0016410, GO:0016740, GO:0016746, GO:0016747, GO:0017076, GO:0030551 +12 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.066 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.12% of strains (170) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
cNMP_binding | PF00027.36 | 4.4e-15 | 40–120 | Cyclic nucleotide-binding domain |
Acetyltransf_3 | PF13302.14 | 1.9e-07 | 156–289 | Acetyltransferase (GNAT) domain |
Acetyltransf_1 | PF00583.32 | 3.1e-06 | 201–280 | Acetyltransferase (GNAT) family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1264 (adenylyl cyclase), high confidence from genomic context alone (score 723 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0997 hyp |
hypothetical protein | 737 | 736 ctx | neighborhood:697 |
Rv0999 hyp |
hypothetical protein | 733 | 733 ctx | neighborhood:732 |
Rv1264 |
adenylyl cyclase | 731 | 723 ctx | cooccurence:554 |
Rv2435c |
cyclase | 651 | 635 | experimental:408 |
Rv2212 |
adenylyl cyclase | 646 | 635 ctx | cooccurence:414 |
Rv3645 |
transmembrane protein | 697 | 611 | |
Rv1625c cya |
adenylate cyclase | 687 | 587 | |
Rv0148 |
short-chain type dehydrogenase/reductase | 585 | 584 | database:431 |
Rv2488c |
LuxR family transcriptional regulator | 584 | 559 | experimental:440 |
Rv1144 |
oxidoreductase | 558 | 558 | database:431 |
Rv1358 |
transcriptional regulator | 583 | 557 | experimental:440 |
Rv0386 |
transcriptional regulator | 582 | 556 | experimental:440 |
Rv1856c |
oxidoreductase | 553 | 552 | database:431 |
Rv1900c lipJ |
lignin peroxidase LipJ | 555 | 535 | |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 569 | 522 | database:431 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acetyltransferase Pat
- MTBC0 PGAP product: GNAT family N-acetyltransferase
- Pfam (hmmscan --cut_ga): cNMP_binding PF00027.36 (E=4e-15), Acetyltransf_3 PF13302.14 (E=2e-07), Acetyltransf_1 PF00583.32 (E=3e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215513.1)
- Domains: Pfam-A via hmmscan --cut_ga — cNMP_binding (PF00027.36), Acetyltransf_3 (PF13302.14), Acetyltransf_1 (PF00583.32)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0664 - Curated reference: UniProt O05581 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
95 functional partner(s); context anchor
Rv1264 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001072|Rv0998| MDGIAELTGARVEDLAGMDVFQGCPAEGLVSLAASVQPLRAAAGQVLLRQGEPAVSFLLISSGSAEVSHVGDDGVAIIARALPGMIVGEIALLRDSPRSATVTTIEPLTGWTGGRGAFATMVHIPGVGERLLRTARQRLAAFVSPIPVRLADGTQLMLRPVLPGDRERTVHGHIQFSGETLYRRFMSARVPSPALMHYLSEVDYVDHFVWVVTDGSDPVADARFVRDETDPTVAEIAFTVADAYQGRGIGSFLIGALSVAARVDGVERFAARMLSDNVPMRTIMDRYGAVWQREDVGVITTMIDVPGPGELSLGREMVDQINRVARQVIEAVG