groEL2 Resolved · high auto-curated
H37Rv Rv0440 · MTBC0 mtbc0_000462 ·
540 aa · 531973–533595 (+) ·
RefSeq NP_214954.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | molecular chaperone GroEL |
|---|---|
| MTBC0 PGAP re-annotation | chaperonin GroEL |
| Revised (this work) | Chaperonin GroEL. Pfam: Cpn60_TCP1 (PF00118.30). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WPE7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Chaperonin GroEL 2 |
| EC (curated) |
EC 5.6.1.7
|
| Curated function | Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding (By similarity). Prevents aggregation of substrate proteins and promotes their refolding. In vitro, activity may be independent of the presence or absence of the GroES co-chaperonin or ATP. Shows weak ATPase activity..; FUNCTION: Mediates association of bacteria with macrophages. Acts as an adhesin that binds CD43. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
O Post-translational modification, protein turnover, chaperones
|
|---|---|
| Preferred name | groL |
| eggNOG description | Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions |
| Orthologous group | COG0459 |
| KEGG orthology |
K04077
|
| KEGG pathways |
map03018, map04212, map04940, map05134, map05152
|
| Gene Ontology (57) |
GO:0001666, GO:0003674, GO:0005488, GO:0005515, GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886 +45 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.038 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 9 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Cpn60_TCP1 | PF00118.30 | 1.2e-75 | 22–522 | TCP-1/cpn60 chaperonin family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: groES (chaperonin GroES), high confidence from genomic context alone (score 998 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3418c groES |
chaperonin GroES | 999 | 998 ctx | cooccurence:772 coexpression:960 experimental:829 textmining:938 |
Rv3417c groEL1 |
chaperonin GroEL | 982 | 982 | coexpression:825 database:900 |
Rv0087 hycE |
formate hydrogenase HycE | 966 | 965 | experimental:965 |
Rv0351 grpE |
stress response protein GrpE | 991 | 948 | coexpression:834 experimental:437 database:455 textmining:851 |
Rv0352 dnaJ1 |
chaperone protein DnaJ | 987 | 901 | coexpression:734 database:493 textmining:880 |
Rv0350 dnaK |
chaperone protein DnaK | 996 | 893 | coexpression:718 experimental:410 textmining:965 |
Rv2373c dnaJ2 |
chaperone protein DnaJ | 935 | 846 | coexpression:556 database:493 textmining:602 |
Rv2299c htpG |
chaperone protein HtpG | 907 | 833 | coexpression:720 experimental:416 textmining:471 |
Rv0312 hyp |
hypothetical protein | 851 | 827 | coexpression:629 experimental:410 |
Rv2264c hyp |
hypothetical protein | 850 | 826 | coexpression:629 experimental:410 |
Rv3446c hyp |
hypothetical protein | 829 | 803 | coexpression:629 experimental:410 |
Rv3433c nnr |
bifunctional ADP-dependent (S)-NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase | 730 | 730 | coexpression:648 |
Rv1310 atpD |
ATP synthase subunit beta | 828 | 706 | database:551 textmining:440 |
Rv3610c ftsH |
zinc metalloprotease FtsH | 773 | 636 | database:404 textmining:404 |
Rv0435c |
ATPase | 655 | 634 | database:401 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: molecular chaperone GroEL
- MTBC0 PGAP product: chaperonin GroEL
- Pfam (hmmscan --cut_ga): Cpn60_TCP1 PF00118.30 (E=1e-75)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214954.1)
- Domains: Pfam-A via hmmscan --cut_ga — Cpn60_TCP1 (PF00118.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0459 - Curated reference: UniProt P9WPE7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
191 functional partner(s); context anchor
groES - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000462|Rv0440|groEL2 MAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPLGLKRGIEKAVEKVTETLLKGAKEVETKEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAVLEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIAEDVEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAILTGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVEGAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPLKQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLAAGVADPVKVTRSALQNAASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF