ceoB Resolved · high auto-curated
H37Rv Rv2691 · MTBC0 - ·
227 aa · 3009344–3010027 (+) ·
RefSeq YP_177900.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | TRK system potassium uptake protein CeoB |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | TRK system potassium uptake protein CeoB. Pfam: TrkA_N (PF02254.25), TrkA_C (PF02080.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
I6XF25
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Trk system potassium uptake protein TrkA |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | ceoB |
| eggNOG description | PFAM TrkA-N domain |
| Orthologous group | COG0569 |
| KEGG orthology |
K03499
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 2.557 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
TrkA_N | PF02254.25 | 1.8e-26 | 3–116 | TrkA-N domain |
TrkA_C | PF02080.27 | 6.4e-09 | 149–212 | TrkA-C domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ceoC (TRK system potassium uptake protein CeoC), high confidence from genomic context alone (score 931 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2692 ceoC |
TRK system potassium uptake protein CeoC | 951 | 931 ctx | neighborhood:882 |
Rv2690c |
integral membrane protein | 919 | 915 ctx | neighborhood:775 cooccurence:633 |
Rv2694c hyp |
hypothetical protein | 692 | 692 ctx | cooccurence:691 |
Rv2689c hyp |
hypothetical protein | 686 | 686 ctx | neighborhood:684 |
Rv1407 fmu |
16S rRNA m5C967 methyltransferase | 671 | 672 | coexpression:574 |
Rv0260c |
transcriptional regulator | 570 | 570 | coexpression:452 |
Rv0511 hemD |
uroporphyrin-III C-methyltransferase | 480 | 480 | coexpression:450 |
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 467 | 465 | coexpression:461 |
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 461 | 462 | coexpression:461 |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 460 | 461 | coexpression:460 |
Rv1631 coaE |
dephospho-CoA kinase CoaE | 448 | 449 | coexpression:417 |
Rv3237c hyp |
hypothetical protein | 510 | 89 | textmining:485 |
Rv1191 hyp |
hypothetical protein | 809 | 65 | textmining:805 |
Rv0428c |
GCN5-like N-acetyltransferase | 732 | 55 | textmining:729 |
Rv1627c |
nonspecific lipid-transfer protein | 804 | 49 | textmining:803 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): TRK system potassium uptake protein CeoB
- Pfam (hmmscan --cut_ga): TrkA_N PF02254.25 (E=2e-26), TrkA_C PF02080.27 (E=6e-09)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177900.1)
- Domains: Pfam-A via hmmscan --cut_ga — TrkA_N (PF02254.25), TrkA_C (PF02080.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0569 - Curated reference: UniProt I6XF25 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
19 functional partner(s); context anchor
ceoC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2691|ceoB MRVVVMGCGRVGASVADGLSRIGHEVAIIDRDSAAFNRLSPQFAGERVLGQGFDRDVLLRAGIQGADAFAAVSSGDNSNIISARLARETFGVPRVVARIYDAKRAEVYERLGIPTITTVPWTTDRLLNALMQDTETAKWRDPTGTVAVAEVVLHEDWVGHRATDLEQATGARIAFLIRFGTGVLPEPKTVLQAGDKVYIAAISGRAAEAAAIAALPPSEDFESGARR