murB Resolved · high auto-curated
H37Rv Rv0482 · MTBC0 mtbc0_000507 ·
369 aa · 573904–575013 (+) ·
RefSeq NP_214996.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | UDP-N-acetylenolpyruvoylglucosamine reductase |
|---|---|
| MTBC0 PGAP re-annotation | UDP-N-acetylmuramate dehydrogenase |
| Revised (this work) | UDP-N-acetylmuramate dehydrogenase. Pfam: FAD_binding_4 (PF01565.29), MurB_C (PF02873.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJL9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UDP-N-acetylenolpyruvoylglucosamine reductase |
| EC (curated) |
EC 1.3.1.98
|
| Curated function | Cell wall formation. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | murB |
| eggNOG description | Cell wall formation |
| Orthologous group | COG0812 |
| EC number |
EC 1.3.1.98
|
| KEGG orthology |
K00075
|
| KEGG pathways |
map00520, map00550, map01100
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 2.644 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FAD_binding_4 | PF01565.29 | 1.4e-15 | 35–166 | FAD binding domain |
MurB_C | PF02873.22 | 2.6e-23 | 230–365 | UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: lprQ (lipoprotein LprQ), high confidence from genomic context alone (score 801 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 993 | 944 | database:900 textmining:888 |
Rv1315 murA |
UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 996 | 908 | database:900 textmining:959 |
Rv0483 lprQ |
lipoprotein LprQ | 824 | 801 ctx | neighborhood:800 |
Rv0481c hyp |
hypothetical protein | 792 | 792 ctx | neighborhood:790 |
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 969 | 727 ctx | cooccurence:512 textmining:892 |
Rv0480c |
amidohydrolase | 695 | 695 ctx | neighborhood:691 |
Rv0479c |
membrane protein | 691 | 691 ctx | neighborhood:690 |
Rv2364c era |
GTPase Era | 596 | 596 ctx | fusion:596 |
Rv2981c ddlA |
D-alanine--D-alanine ligase | 872 | 595 | coexpression:441 textmining:699 |
Rv2151c ftsQ |
cell division protein FtsQ | 695 | 555 | coexpression:493 |
Rv2973c recG |
ATP-dependent DNA helicase RecG | 531 | 531 ctx | cooccurence:430 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 936 | 506 | textmining:877 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 954 | 440 | textmining:922 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 934 | 416 | textmining:893 |
Rv0079 hyp |
hypothetical protein | 410 | 411 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: UDP-N-acetylenolpyruvoylglucosamine reductase
- MTBC0 PGAP product: UDP-N-acetylmuramate dehydrogenase
- Pfam (hmmscan --cut_ga): FAD_binding_4 PF01565.29 (E=1e-15), MurB_C PF02873.22 (E=3e-23)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214996.1)
- Domains: Pfam-A via hmmscan --cut_ga — FAD_binding_4 (PF01565.29), MurB_C (PF02873.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0812 - Curated reference: UniProt P9WJL9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
53 functional partner(s); context anchor
lprQ - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000507|Rv0482|murB MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGADRPLVFAGGSNLVIAENLTDLTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCTGEVRWVSARDLRFGYRTSVLKHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRARKGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWLVERAGFGKGYPDAGAAPCRLSTKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKPEPVLIGCML