plsB1 Resolved · high auto-curated
H37Rv Rv1551 · MTBC0 mtbc0_001657 ·
621 aa · 1765256–1767121 (+) ·
RefSeq NP_216067.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyltransferase PlsB |
|---|---|
| MTBC0 PGAP re-annotation | lysophospholipid acyltransferase |
| Revised (this work) | Lysophospholipid acyltransferase. Pfam: Acyltransferase (PF01553.27), GPAT_C (PF19277.5). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WI59
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative acyltransferase plsB1 |
UniProt still lists this protein as Putative acyltransferase plsB1; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| Preferred name | plsB1 |
| eggNOG description | Belongs to the GPAT DAPAT family |
| Orthologous group | COG2937 |
| EC number |
EC 2.3.1.15
|
| KEGG orthology |
K00631
|
| KEGG pathways |
map00561, map00564, map01100, map01110
|
| KEGG modules |
M00089
|
| Gene Ontology (8) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0016020, GO:0030312, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.482 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 11 missense, 0 nonsense, 3 frameshift |
| Disruption | 3 distinct premature-stop/frameshift site(s); most common in 26.26% of strains (38129) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acyltransferase | PF01553.27 | 5.2e-09 | 106–241 | Acyltransferase |
GPAT_C | PF19277.5 | 3.0e-69 | 252–586 | Glycerol-3-phosphate acyltransferase C-terminal region |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fadD11 (fatty-acid--CoA ligase FadD11), high confidence from genomic context alone (score 971 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1550 fadD11 |
fatty-acid--CoA ligase FadD11 | 972 | 971 ctx | neighborhood:812 coexpression:853 |
Rv2482c plsB2 |
glycerol-3-phosphate acyltransferase | 926 | 922 | database:900 |
Rv2982c gpdA2 |
glycerol-3-phosphate dehydrogenase | 940 | 904 | database:900 textmining:409 |
Rv0564c gpdA1 |
glycerol-3-phosphate dehydrogenase | 940 | 904 | database:900 textmining:409 |
Rv2182c |
1-acylglycerol-3-phosphate O-acyltransferase | 908 | 904 | database:900 |
Rv3302c glpD2 |
glycerol-3-phosphate dehydrogenase | 915 | 902 | database:900 |
Rv2249c glpD1 |
glycerol-3-phosphate dehydrogenase | 907 | 902 | database:900 |
Rv1692 |
phosphatase | 900 | 901 | database:900 |
Rv3696c glpK |
glycerol kinase | 913 | 900 | database:900 |
Rv1549 fadD11.1 |
Possible fatty-acid-CoA ligase FadD11.1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Rv1549, (MTCY48.16c), len: 175 aa. Possible f | 843 | 835 ctx | neighborhood:771 |
Rv0104 hyp |
hypothetical protein | 770 | 762 | coexpression:762 |
Rv2483c plsC |
bifunctional L-3-phosphoserine phosphatase/1-acyl-sn-glycerol-3-phosphate acyltransferase | 821 | 711 ctx | cooccurence:664 textmining:407 |
Rv2484c |
diacyglycerol O-acyltransferase | 772 | 694 ctx | cooccurence:649 |
Rv1552 frdA |
fumarate reductase flavoprotein subunit | 692 | 692 ctx | neighborhood:439 coexpression:474 |
Rv0441c hyp |
hypothetical protein | 697 | 684 | database:549 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acyltransferase PlsB
- MTBC0 PGAP product: lysophospholipid acyltransferase
- Pfam (hmmscan --cut_ga): Acyltransferase PF01553.27 (E=5e-09), GPAT_C PF19277.5 (E=3e-69)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216067.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acyltransferase (PF01553.27), GPAT_C (PF19277.5)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2937 - Curated reference: UniProt P9WI59 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
55 functional partner(s); context anchor
fadD11 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001657|Rv1551|plsB1 MTAREVGRIGLRKLLQRIGIVAESMTPLATDPVEVTQLLDARWYDERLRALADELGRDPDSVRAEAAGYLREMAASLDERAVQAWRGFSRWLMRAYDVLVDEDQITQLRKLDRKATLAFAFSHRSYLDGMLLPEAILANRLSPALTFGGANLNFFPMGAWAKRTGAIFIRRQTKDIPVYRFVLRAYAAQLVQNHVNLTWSIEGGRTRTGKLRPPVFGILRYITDAVDEIDGPEVYLVPTSIVYDQLHEVEAMTTEAYGAVKRPEDLRFLVRLARQQGERLGRAYLDFGEPLPLRKRLQEMRADKSGTGSEIERIALDVEHRINRATPVTPTAVVSLALLGADRSLSISEVLATVRPLASYIAARNWAVAGAADLTNRSTIRWTLHQMVASGVVSVYDAGTEAVWGIGEDQHLVAAFYRNTAIHILVDRAVAELALLAAAETTTNGSVSPATVRDEALSLRDLLKFEFLFSGRAQFEKDLANEVLLIGSVVDTSKPAAAADVWRLLESADVLLAHLVLRPFLDAYHIVADRLAAHEDDSFDEEGFLAECLQVGKQWELQRNIASAESRSMELFKTALRLARHRELVDGADATDIAKRRQQFADEIATATRRVNTIAELARRQ