murA Resolved · high auto-curated
H37Rv Rv1315 · MTBC0 mtbc0_001407 ·
418 aa · 1479349–1480605 (+) ·
RefSeq NP_215831.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
| Revised (this work) | UDP-N-acetylglucosamine 1-carboxyvinyltransferase. Pfam: EPSP_synthase (PF00275.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJM1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
| EC (curated) |
EC 2.5.1.7
|
| Curated function | Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | murA |
| eggNOG description | Belongs to the EPSP synthase family. MurA subfamily |
| Orthologous group | COG0766 |
| EC number |
EC 2.5.1.7
|
| KEGG orthology |
K00790
|
| KEGG pathways |
map00520, map00550, map01100
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.349 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
EPSP_synthase | PF00275.26 | 8.5e-124 | 7–406 | EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1314c (cob(I)yrinic acid a,c-diamide adenosyltransferase), high confidence from genomic context alone (score 786 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1018c glmU |
bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase | 977 | 921 | database:900 textmining:730 |
Rv0482 murB |
UDP-N-acetylenolpyruvoylglucosamine reductase | 996 | 908 | database:900 textmining:959 |
Rv1314c |
cob(I)yrinic acid a,c-diamide adenosyltransferase | 786 | 786 ctx | neighborhood:785 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 987 | 780 ctx | cooccurence:727 textmining:948 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 963 | 750 ctx | cooccurence:735 textmining:861 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 908 | 749 ctx | cooccurence:733 textmining:649 |
Rv2154c ftsW |
lipid II flippase FtsW | 893 | 720 ctx | cooccurence:572 textmining:633 |
Rv3915 cwlM |
peptidoglycan hydrolase | 908 | 719 ctx | cooccurence:701 textmining:686 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 837 | 693 ctx | cooccurence:680 textmining:494 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 966 | 654 ctx | cooccurence:613 textmining:908 |
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 976 | 638 ctx | cooccurence:606 textmining:937 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 975 | 626 ctx | cooccurence:591 textmining:937 |
Rv0017c rodA |
cell division protein RodA | 708 | 554 | |
Rv3859c gltB |
glutamate synthase large subunit | 654 | 546 ctx | neighborhood:544 |
Rv1307 atpH |
ATP synthase subunit b/delta | 573 | 530 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
- MTBC0 PGAP product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
- Pfam (hmmscan --cut_ga): EPSP_synthase PF00275.26 (E=9e-124)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215831.1)
- Domains: Pfam-A via hmmscan --cut_ga — EPSP_synthase (PF00275.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0766 - Curated reference: UniProt P9WJM1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
64 functional partner(s); context anchor
Rv1314c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001407|Rv1315|murA MAERFVVTGGNRLSGEVAVGGAKNSVLKLMAATLLAEGTSTITNCPDILDVPLMAEVLRGLGATVELDGDVARITAPDEPKYDADFAAVRQFRASVCVLGPLVGRCKRARVALPGGDAIGSRPLDMHQAGLRQLGAHCNIEHGCVVARAETLRGAEIQLEFPSVGATENILMAAVVAEGVTTIHNAAREPDVVDLCTMLNQMGAQVEGAGSPTMTITGVPRLHPTEHRVIGDRIVAATWGIAAAMTRGDISVAGVDPAHLQLVLHKLHDAGATVTQTDASFRVTQYERPKAVNVATLPFPGFPTDLQPMAIALASIADGTSMITENVFEARFRFVEEMIRLGADARTDGHHAVVRGLPQLSSAPVWCSDIRAGAGLVLAGLVADGDTEVHDVFHIDRGYPLFVENLVSLGAEIERVCC