Rv3327 Family assigned · medium auto-curated
H37Rv Rv3327 · MTBC0 mtbc0_000846 ·
358 aa · 893455–894528 (+) ·
RefSeq NP_217844.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transposase fusion protein |
|---|---|
| MTBC0 PGAP re-annotation | IS110-like element IS1547 family transposase |
| Revised (this work) | IS110 family transposase. Pfam: DEDD_Tnp_IS110 (PF01548.24), Transposase_20 (PF02371.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53377
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable transposase fusion protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| eggNOG description | Transposase IS116 IS110 IS902 |
| Orthologous group | COG3547 |
| KEGG orthology |
K07486
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.4 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DEDD_Tnp_IS110 | PF01548.24 | 1.0e-23 | 4–155 | Transposase |
Transposase_20 | PF02371.23 | 5.4e-22 | 222–307 | Transposase IS116/IS110/IS902 family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3326 (Probable transposase; Rv3326, (MTV016.26), len: 328 aa. Probable transposase for insertion element IS6110. Identical to many other M. tuberc), medium confidence from genomic context alone (score 632 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3326 |
Probable transposase; Rv3326, (MTV016.26), len: 328 aa. Probable transposase for insertion element IS6110. Identical to many other M. tuberc | 633 | 632 ctx | neighborhood:610 |
Rv3325 |
Rv3325, (MTV016.25), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase sub | 612 | 611 ctx | neighborhood:610 |
Rv1286 cysC |
adenylyl-sulfate kinase | 475 | 475 | coexpression:475 |
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 458 | 458 ctx | neighborhood:449 |
Rv2807 hyp |
hypothetical protein | 457 | 458 | coexpression:441 |
Rv3322c |
methyltransferase | 460 | 457 ctx | neighborhood:449 |
Rv3324c moaC3 |
cyclic pyranopterin monophosphate synthase accessory protein | 454 | 454 ctx | neighborhood:446 |
Rv3324A |
Rv3324A, len: 44 aa. Probable pseudogene moaB3,fragment of pterin-4-alpha-carbinolamine dehydratase,equivalent to C-terminus of MT3426|Q8VJ3 | 450 | 449 ctx | neighborhood:446 |
Rv0513 |
transmembrane protein | 421 | 422 ctx | cooccurence:418 |
Rv2399c cysT |
sulfate ABC transporter permease CysT | 409 | 409 | coexpression:409 |
Rv0433 |
carboxylate-amine ligase | 405 | 406 | coexpression:406 |
Rv0395 hyp |
hypothetical protein | 503 | 93 | textmining:475 |
Rv2813 hyp |
hypothetical protein | 811 | 90 | textmining:801 |
Rv1758 cut1 |
cutinase | 532 | 84 | textmining:511 |
Rv1777 cyp144 |
cytochrome P450 Cyp144 | 641 | 70 | textmining:630 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transposase fusion protein
- MTBC0 PGAP product: IS110 family transposase
- Pfam (hmmscan --cut_ga): DEDD_Tnp_IS110 PF01548.24 (E=1e-23), Transposase_20 PF02371.23 (E=5e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217844.1)
- Domains: Pfam-A via hmmscan --cut_ga — DEDD_Tnp_IS110 (PF01548.24), Transposase_20 (PF02371.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3547 - Curated reference: UniProt O53377 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
19 functional partner(s); context anchor
Rv3326 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000846|Rv3327| MVVVGTDAHKYSHTFVATDEVGRQLGEKTVKATTAGHATAIMWAREQFGLELIWGIEDCRNMSARLERDLLAAGQQVVRVPTKLMAQTRKSARSRGKSDPIDALAVARAVMRETDLPLATHDETSRELKLLTDRRDVLVAQRTSAINRLRWLVHELDPERAPAARSLDAAKHQQALRTWLDTQPGLVAELARAELTDIIRLTGEINTLAQRISARVHQVAPALLEIPGCAELTAAKIVGEAAGVTRFKSEAAFACHAAVAPIPVWSGNTAGQMRLSRSGNRQLNAALHRIALTQIRMTDSRGQAYYQRLQDAGKTKRAALRCLKRRLARTVFQALRTVHQPSSEHTQPAAACHRSYCS