Rv2917 Family assigned · medium auto-curated
H37Rv Rv2917 · MTBC0 - ·
626 aa · 3226363–3228243 (+) ·
RefSeq NP_217433.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Contains ResIII (PF04851.22), DEAD (PF00270.36), SWI2_SNF2 (PF18766.8) domain(s); putative function inferred from the domain architecture. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WL21
SwissProt · reviewed
· Predicted
|
|---|---|
| UniProt name | Uncharacterized protein Rv2917 |
UniProt still lists this protein as Uncharacterized protein Rv2917; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| Preferred name | sdrA |
| eggNOG description | Type III restriction enzyme, res subunit |
| Orthologous group | COG1061 |
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.481 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ResIII | PF04851.22 | 1.5e-15 | 65–218 | Type III restriction enzyme, res subunit |
DEAD | PF00270.36 | 3.0e-08 | 69–217 | DEAD/DEAH box helicase |
SWI2_SNF2 | PF18766.8 | 7.2e-08 | 89–274 | SWI2/SNF2 ATPase |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1329c dinG |
ATP-dependent helicase DinG | 887 | 879 | experimental:685 database:622 |
Rv0667 rpoB |
DNA-directed RNA polymerase subunit beta | 855 | 853 | experimental:578 database:621 |
Rv3457c rpoA |
DNA-directed RNA polymerase subunit alpha | 852 | 853 | experimental:627 database:621 |
Rv1390 rpoZ |
DNA-directed RNA polymerase subunit omega | 847 | 847 | experimental:613 database:621 |
Rv1629 polA |
DNA polymerase I | 786 | 766 | experimental:430 database:580 |
Rv2033c hyp |
hypothetical protein | 754 | 755 ctx | cooccurence:752 |
Rv2090 |
5'-3' exonuclease | 765 | 752 | experimental:430 database:580 |
Rv0668 rpoC |
DNA-directed RNA polymerase subunit beta' | 747 | 686 | database:620 |
Rv2529 hyp |
hypothetical protein | 705 | 686 | database:610 |
Rv2101 helZ |
helicase HelZ | 692 | 669 | database:615 |
Rv3258c hyp |
hypothetical protein | 660 | 660 ctx | cooccurence:660 |
Rv2256c hyp |
hypothetical protein | 654 | 654 ctx | cooccurence:654 |
Rv1334 mec |
[CysO | 629 | 629 | database:613 |
Rv3036c TB22.2 hyp |
hypothetical protein | 620 | 621 | database:563 |
Rv3812 PE_PGRS62 |
PE-PGRS family protein PE_PGRS62 | 620 | 621 | database:563 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): hypothetical protein
- Pfam (hmmscan --cut_ga): ResIII PF04851.22 (E=1e-15), DEAD PF00270.36 (E=3e-08), SWI2_SNF2 PF18766.8 (E=7e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217433.1)
- Domains: Pfam-A via hmmscan --cut_ga — ResIII (PF04851.22), DEAD (PF00270.36), SWI2_SNF2 (PF18766.8)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1061 - Curated reference: UniProt P9WL21 (SwissProt, reviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 50 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2917| MRVTRLVDAESTRCDVGPAPKSVAMLHFTAATSRFRLGRERANSVRSDGGWGVLQPVSATFNPPLRGWQRRALVQYLGTQPRDFLAVATPGSGKTSFALRIAAELLRYHTVEQVTVVVPTEHLKVQWAHAAAAHGLSLDPKFANSNPQTSPEYHGVMVTYAQVASHPTLHRVRTEARKTLVVFDEIHHGGDAKTWGDAIREAFGDATRRLALTGTPFRSDDSPIPFVSYQPDADGVLRSQADHTYGYAEALADGVVRPVVFLAYSGQARWRDSAGEEYEARLGEPLSAEQTARAWRTALDPEGEWMPAVITAADRRLRQLRAHVPDAGGMIIASDRTTARAYARLLTTMTAEEPTVVLSDDPGSSARITEFAQGTSRWLVAVRMVSEGVDVPRLSVGVYATNASTPLFFAQAIGRFVRSRRPGETASIFVPSVPNLLQLASALEVQRNHVLGRPHRESAHDPLDGDPATRTQTERGGAERGFTALGADAELDQVIFDGSSFGTATPTGSDEEADYLGIPGLLDAEQMRALLHRRQDEQLRKRAQLQKGATQPATSGASASVHGQLRDLRRELHTLVSIAHHRTGKPHGWIHDERRRRCGGPPIAAATRAQIKARIDALRQLNSERS